Preparation And Application Of Composite Nano-Fiber Membranes Containing Lappaconition Hydrobromide/Zinc Oxide By Electrospinning

Electrospinning drug loading system combines electrospinning technology and drug release.Drugs can be uniformly dispersed in polymer fibers through electrostatic spinning.The low dose and the sudden release of drugs in the human body have attracted more and more attention.There are many types of polymers used as controlled release systems,both biodegradable and non-biodegradable,but these polymers must be biocompatible and cannot cause rejection in the human body.Spinning some biopolymer materials through the electrostatic spinning process to prepare nanofibers can effectively improve the polymer's degradability and biocompatibility,and reduce the probability of rejection reactions.In general,the smaller the size of the drug and its packaging material,the better the body will absorb the drug,because the dissolution rate of the drug increases with the surface area of the drug and the carrier.In this paper,an electrostatic spinning device is used to dissolve PLA and PLA in dichloromethane for electrostatic spinning.The spinning solution mass fraction,PLA/PCL mass ratio,voltage,distance,flow rate and other process parameters have been studied.The effect of the morphology and structure of the nanofibers was obtained,and the optimal spinning process parameters were obtained according to the orthogonal test.Electrospinning was performed to prepare PLA/PCL composite nanofibers.The chemical structure of PLA/PCL composite nanofibers was studied using infrared spectroscopy(IR),crystallinity(XRD),and contact angle tests;ZnO was miscible with PLA/PCL In the spinning solution,blended electrostatic spinning was performed to prepare PLA/PCL/ZnO composite nanofiber membranes and tested for antibacterial properties.PLA/PCL/ZnO composite nanofibers and PLA/PCL/ZnO drug-loaded composites were prepared separately.Nanofibers were used to study the drug's sustained-release effect.The UV-spectrophotometer was used to test and analyze the drug release properties and behaviors of fibers in a simulated body fluid at 37°C.ZnO was grafted with PLA/PCL composite nanometers by chemical crosslinking.On the fiber,To cross-linked PLA/PCL/ZnO composite nanofiber membranes and test their antibacterial properties;blended PLA/PCL/ZnO composite nanofiber membranes and cross-linked PLA/PCL/ZnO composite nanofiber membranes with different ZnO concentrations Cytotoxicity was performed and the results obtained were analyzed statistically.The thesis mainly includes the following parts:(1)The first is to explore the best process of PLA/PCL composite nanofiber membrane.Mainly explore the PLA/PCL as a carrier to prepare the drug release system,and study the effects of different PLA,PCL blending ratios and different voltages,distances,and flow rates on fiber diameter and morphology.Finally,they passed the orthogonal test.The design has determined the best spinning conditions: the electrostatic field voltage is 8 kV,the drum receiving distance is 6 cm,and the flow rate of the electrostatic spinning solution is 2 mL/h.The diameter distribution range of the composite nanofiber membranes prepared under these conditions is small,between 2-8?m.(2)Secondly,ZnO was mixed into the PCL/PLA electrospinning solution for spinning to obtain the blended PLA/PCL/ZnO composite nanofibers,and the chemical structure characterization and performance research were performed.It mainly includes SEM,XRD,FT-IR,contact angle test,antibacterial performance test,drug sustained release performance test.(3)ZnO was grafted onto PLA/PCL composite nanofibers by chemical cross-linking method to obtain cross-linked PLA/PCL/ZnO composite nanofiber membranes,and the chemical structure characterization and performance research were performed on them.It mainly includes SEM,XRD,FT-IR,contact angle test,antibacterial performance test,drug sustained release performance test.(4)Cytotoxicity was performed on blended PLA/PCL/ZnO composite nanofiber membranes and cross-linked PLA/PCL/ZnO composite nanofiber membranes with different ZnO concentrations.Statistical analysis was performed on the results obtained.