| With the development of microscopy technology,the optical system has higher and higher requirements for the illumination uniformity of the specimen surface.Therefore,the microscope illumination system has also evolved from the early mirror to the current critical illumination,Kola illumination and the like.In the microscopic observation,the required illumination color temperature is often different depending on the observation object,that is,the light source of different color temperatures has different color development capabilities for different sample specimens.Most of the existing microscopes still change the color temperature of the specimen surface by replacing the illumination source.Therefore,the continuous adjustment of the color temperature of the microscope illumination source has become a subject of research by microscope companies all over the world.This thesis proposes three kinds of microscope illumination methods:WRGB integrated LED illumination,Dual color temperature LED illumination and red,green and cool white three LED mixed illumination illumination through the basic principles of colorimetry.And the application of the four light mixing methods of dichroic filter,light mixing column,frosted lens and fly-eye lens in the above micro-illumination is discussed.And through the Lighttools optical software,the red,green,cool white three LEDs and the spectral separation film combination,the WRGB four-color LED and the light mixing column combination,the Dual color temperature LED and the frosted lens and the fly-eye lens are respectively combined into three combinations.The micro-lighting design was optimized for simulation.After considering the cost control of the micro-light source and the replaceability of the existing monochromatic warm white LED light source,the Dual color temperature LED micro-illumination was selected as the subsequent optimization direction.The Dual color temperature LED will appear in the observation field under the low magnification objective lens,and the cross-distribution of cold and warm color temperature will appear.Therefore,two solutions of frosted lens and fly-eye lens are proposed to optimize the mixed light effect of the Dual color temperature LED,and the surface of the specimen under the low magnification objective lens is solved.The problem of uneven color temperature distribution caused by the cross-distribution of cold and warm color temperature.Through the reasonable simulation optimization and the comparison of the platform test,the simulated fly-eye lens scheme and the frosted lens scheme can eliminate the uneven color temperature distribution under the low magnification objective lens.The experimental results show that both the fly-eye lens scheme and the frosted lens scheme have a certain degree of loss of light energy,but the illumination uniformity CV test values of both the Dual color temperature LEDs after homogenization are better than the original monochromatic temperature LED illumination.The test value is small,that is,the uniformity of the surface illumination of the specimen is better.Compared with the frosted lens system and the fly-eye lens system,the two have their own advantages and disadvantages.The frosted lens system is better than the fly-eye lens system in terms of uniform light mixing,and its light energy utilization rate is about 10%smaller than that of the fly-eye lens system.To meet the brightness requirements of the microscope’s field of vision,the frosted lens system is the first choice for mixed light homogenization systems.However,in microscopes that require high brightness,a fly-eye lens system can still be selected.The light emitted from the frosted surface is concentrated at a certain angle after being concentrated by the convex surface of the lens,which has a certain effect on the utilization of light energy,the experimental test results show that the matte lens in this paper has a nearly 65%improvement in light energy utilization compared to the same frosted sanding board. |
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