Research And Application On Fluorescent Microscope Based On Monochrome LED

Fluorescence in situ hybridization(FISH),as an important detection method in molecular biology,has important clinical significance in early diagnosis and precise treatment of diseases.Therefore,accurate detection results are an important basis to realize its clinical significance.The whole FISH experiment included pretreatment,hybridization,microscopic examination and other major steps,among which microscopic examination required the use of fluorescence microscope.Fluorescence microscope is an efficient and simple observation tool and has become one of the most important tools in biomedical field.Through to the research situation at home and abroad research background and white FISH detection experiment,this topic to present accurate FISH detection results as the goal,this paper proposes a fluorescence microscope system design scheme based on single color LED light source,through to the LED light source,light source drive,study and analysis of the key technologies such as image enhancement algorithm,determine the final solution.At the same time,the fluorescent microscope of the monochrome LED light source was applied in FISH test of white blood cells,and a good experimental result was obtained.The main research work of this topic is mainly divided into the following three parts:Firstly,by comparing the photoelectric characteristics of LED,metal halide lamp and high-pressure mercury lamp,the scheme of LED as monochrome light source of fluorescence microscope was determined.According to the white blood cell FISH test requirements adopted in this subject,the optical and electrothermal characteristics of LED were analyzed to ensure that the selected LED performance fully meets the design needs.And carry on the design and selection to other related optical elements,finally completes the light source structure design together.Secondly,according to the voltage and current determined by LED flux and other parameters,a corresponding driving power supply was designed for the monochrome light source,and the designed LED light source system was compared with the original mercury lamp of the microscope to verify the feasibility of the LED light source systemAt last,this subject studied the image enhancement algorithm.In the FISH experiment using LED monochrome light source through the fluorescence microscope which has completed the automatic focusing system,the sample image was dark around the light center and the signal point could not be seen clearly due to the LED itself.According to the characteristics of FISH images,an algorithm of preprocessing layered images and then enhancing the signals was proposed.And through the MATLAB software to complete color image decomposition channel;Delete the pictures do not want part;(3)divide the cell area and thebackground area;(4)filling holes;(5)corrosion and expansion;The cell background picture matting operation;(7)adaptive enhancement of the luminous region;We carried out simulation verification on the sample image by combining pictures and other steps,which improved the accuracy of LED based fluorescence microscopy.