Studies On The Large Scale Cultivation Of A Green Alga Chlorococcum Pamirum Under Outdoor Conditions

With the rapid development of global economy and the increasing demand of fossil oil,oil,natural gas and other resources are facing the threat of being exhausted.On the other hand,a large number of fossil energy use and emit a lot of CO₂,causing the greenhouse effect.So looking for new sources of energy is harmless to the environment of sustainable utilization is the key to solving the problem.And the microalgae biomass for preparing diesel oil plants compared with the traditional,has more prominent advantages,has attracted more and more attention of researchers,is one of the promising research directions.The ability of C.pamirum was studied in this paper and the possibility of outdoor large-scale cultivation of oil in the four seasons of the accumulation of.A thorough study on it from different angles and different thickness of the bioreactor,inoculation density.And then studied under the same inoculation density,nitrogen deficiency in culture medium,effect of different photobioreactor thickness on the growth and physiology of C.pamirum.Large scale cultivation of the four seasons in the outdoor experimental results show that:C.pamirum can adapt to different seasonal outdoor climate,even if it is summer in general intensity of 800-2000(mol m^-2s^-1),algae can grow very well,in the fall with the highest average growth rate of 0.174(D^-1)and the average biomass maximum productivity of 64.41?mgL-1d-1?,followed by the spring and summer,is the lowest in winter.In different initial density,the lowest density of algal cells inoculated into 0.1has a maximum specific growth rate,but the biomass productivity,it is not the highest,is the highest density of algal cells was 0.4.This shows that the specific growth rate and biomass productivity is not necessarily proportional relationship.In the photo bioreactor reactor thickness,thin in the growth of algae was significantly better than the photobioreactor thickness.In terms of oil accumulation,The growth of algae oil better accumulated less fat content,winter is the highest?34.8%?,followed by summer?30.1%?,the last is the spring?27.96%?and autumn?25.61%?,contrary to the growth of their.In the flat plate photobioreactors of different thickness effects on C.pamirum experiment in the autumn and winter had a fall,the research mainly focuses on the deficiency in nitrogen environment,ability,growth and lipid accumulation of algae shows,photo bioreactor in autumn than the growth of thin rate?0.1d-1?than the photobioreactor thickness in 2cm,has a maximum biomass productivity(35.6mgL^-1day^-1),5cm has the largest oil content?51.8%?.In the winter study,not only to the growth of algae was studied,also the physiological do research.Growth is thin,light biological reactor with different advantages,the optical bioreactor 5cm not only had the highest lipid content?38.4%?,there is a maximum biomass productivity(28.81 mg L^-1 d^-1).This shows that in the outdoor culture can satisfy both the increase of biomass and lipid content increased,the.In the physical aspects,by photo bioreactor in thin algae light intensity will be greater,is favorable for photosynthesis,but the intensity is higher than a certain time,may induce photoinhibition,then affect cell growth and lipid accumulation.The experimental results show that,algae photobioreactor growth than in thin cell reactor in the thick growth faster,but by the reactor and thin in photoinhibition phenomenon will exist,so the reactor growth and lipid accumulation in this experiment the optimum thickness of 5cm.The above results showed that Chlorococcum,pamirum is the production of bio diesel has great potential of microalgae,can adapt to the situation of the four seasons,large-scale farming in the outdoor.To further optimize the photo bioreactor conditions,the algae will be studied and better economic value.