| Sinojackia rehderiana belongs to Styracaceae.It is an endangered species,endemic to the subtropical area of China.It mainly distributes in northern Guangdong,Hunan,and Jiangxi province.In the article,S.rehderiana population distributed in Yongxiu county was studied,and some fruit variation was founded.Using numerical classification analysis of fruits and flowers on morphology,and ISSR molecular markers to study its genetic diversity,the fruit variation in the population was explored.The flowers phenotypic data analysis showed that significant differences were existed on the corolla longitudinal diameter,the number of stamens,the anther length,and the diameter of the ovary.The order of variation coefficient of phenotypic characters was calyx length?17.58%?>bud vertical diameter?12.87%?>calyx wide?12.56%?>ovary length?11.53%?>bud lateral diameter?10.05%?>anther length?9.61%?>ovary diameter?9.49%?>stamen number?8.72%?.The CV order among 4 fruit types was big tilt type?10.85%?<big long type?11.42%?<big short type and narrow type?11.61%?.Anther length and bud vertical diameter,bud vertical diameter and bud lateral diameter,bud vertical diametere and ovary length,bud lateral diameter and ovary length were significantly correlated.Cluster analysis divided the population into three groups,they were big short type,narrow type and big tilt type,and bit long type.Principal component analysis showed that big short type subpopultion was separated from big long type subpopultion at principal component 1.Big tilt type subpopulation obviously separated from big long type subpopulation and big short subpopulation at principal component 2,only narrow type and big tilt type mixed together.The fruits phenotypic data analysis showed that all fruit phenotypic traits of S.rehderiana were significantly different between subpopulations.The order of variation coefficient of phenotypic characters was beak length?30.68%?>fruit weight?25.85%?>length-width ratio?13.69%?>fruit width?11.58%?>fruit length?11.55%?>bending?3.30%?.The CV order among 4 subpopulations was big short type?14.25%?<big long type?16.34%?<big tilt type?16.82%?<narrow type?17.03%?.Fruit width and fruit weight,length-width ratio and fruit width,fruit length and beak length,fruit length and length-width ratio were significantly correlated.Cluster analysis divided the population into five groups,they were big short type,big long type,big tilt type,narrow type and mixed type of big tilt type and narrow type.Principal component analysis showed that narrow type subpopulation was obviously separated from other three subpopultions.Big short type subpopulation separated from big long type subpopulation and big tilt subpopulation,only big long type and big tilt type mixed together.Genetic diversity analysis:optimization of ISSR reaction system:template DNA 50ng,primer concentration 0.6?mol·L-1,the primer annealing temperature52?to 57?.85 discernible DNA fragments of which 78?87.5%?were polymorphic.The total observed number of alleles?Na?of three groups was 1.9176,the effective number of alleles?Ne?was 1.5779,Nei's genetic diversity index?h?was 0.3323,Shannon diversity index?I?was 0.4934,the percentage of polymorphism loci?PPB?was 91.8%.Differentiation coefficient among groups?Gst?was 0.0696,it showed that most genetic variation?93.04%?was resided among individuals within subpopulations.Gene flow?Nm?was 6.6856.AMOVA analysis showed that the variation between groups was 8.43%,the variation within group was91.58%.The range of genetic distance was between 0.0475-0.0559,the range of genetic identity was between 0.9456-0.9536.UPGMA cluster analysis divided the population into four groups:big short type group,big tilt type group,narrow type group and big short type.The variation of fruits and clustering results were in accordance with their geographical distribution.The narrow type subpopulation and big short type subpopulation are located respectively on both edges of the sample area,big tilt type and big long type are located in the middle transition zone.S.rehderiana has good adaptability and wide range of natural distribution.Some phenotypic variation was resulted from genomic hybridization because of high gene flow existed in the breeding.Based on correlation analysis between phenotypic variation and genetic diversity,the fruit phenotypic variation was mainly controlled by environmental factors,genetic factors played a secondary role. |
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