Genetic Diversity Analysis Of Chimonobambusa Hejiangensis Based On ISSR Markers

Chimonobambusa hejiangensis belongs to the subfamily Bambusoideae,Poaceae,which is an important taxa unique found in Sichuan and Guizhou Province,south-western China.It is regarded as a special bamboo for bamboo shoots in autumn.There has been no research on genetic diversity of the populations in the original producing area of Ch.hejiangensis to this day.In order to provide some theoretical references for the cultivation,utilization,breeding and protection of Ch.hejiangensis in the producing area.This paper used Inter-sequence simple repeats(ISSR)markers technique,to analyze the genetic diversity of ten populations of Ch.hejiangensis from Chishui county in Guizhou Province and three county in Sichuan Province.The main results are as follows.In this experiment,11 ISSR primers were used to amplify Ch.hejiangensis DNA,and151 bands were amplified,among which 130 were polymorphic,with a polymorphism ratio of86.1%.On average,13.7 bands were amplified from each primer,and 11.8 polymorphic bands were amplified from each primer.The number of alleles was 1.35-1.90,with an average of1.71.The number of effective alleles ranged from 1.0751 to 1.3618,with an average of1.2645.The average heterozygous degree ranged from 0.0578 to 0.2405,with an average of0.1729.Shannon's diversity index is between 0.1030 and 0.3857,with an average of0.2782.The average heterozygosity H of Ch.hejiangensis was 0.1729,and the average value of Shannon's diversity index was 0.2782,indicating that the level of genetic diversity of species was low.In the same region,because the difference of altitude and the intensity of human activities,the genetic diversity of population is different.Generally,the genetic diversity of Ch.hejiangensis varies with the change of altitude,showing a positive correlation.Among the10 selected populations,the average heterozygosity and Shannon's diversity index of BY 1population were the highest,followed by ELB population.Despite the high degree of genetic differentiation,the main genetic differentiation is still within the population,accounting for61.67%.The low gene flow indicates that there is little gene exchange among differentpopulations of Ch.hejiangensis,and the level of gene differentiation among populations is low.UPGMA method was used to construct cluster UPGMA dendrogram of population of Ch.hejiangensis genetic relationship by NTSYS 2.1 software.The cluster map of 10 populations can be divided into three branches when the genetic similarity coefficient is 0.88.FB population is a single branch;DS population,LHK population and BY 3 population are clustered into one branch;BY 1 population,HJ population,SW population are clustered into a small branch and then with CQ population,ELB population and BY 2 population form a large branch containing six populations.