| Tea(Camellia sinensis(L.)O.Kuntze)is a kind of natural health beverage which played an extremely important role in drinking history in the world.This research showed that the differences of rare earth,F and Al element content in different tea cultivars(lines)reached a significant level based on the perspective of germplasm resources.Then the effects of tea mowing height on the accumulation,the differentially expressed genes and its metabolic pathway of 3 different tea varieties(lines)using RNA-seq technology were researched in order to study the accumulation characteristics of rare earth,F and Al and the internal mechanism which caused the difference among the varieties.These research results has a great scientific significance at screening absorption of tea germplasm enrichment.The main results are listed as follows:(1)In this paper we collected a bud and three leaves of the tea varieties as materials under two different natural environmental conditions,Anxi and Wuyishan in spring,summer,autumn,.Using ICP-MS method for the determination of rare earth elements and Al content in tea,fluorine ion electrode method to determine the F content in tea plant with spss19.0 for statistical analysis and cluster analysis.The study showed that different tea varieties(lines)between REE,F and Al in different seasons,different habitat conditions are significantly different,low enriched/accumulation REE,F and Al cultivars(lines)can maintain relatively stable heritablely between seasons,it exists absorption of certain laws.This assay screened high,medium and low three levels enrichment of aluminum and fluoride elements from the tea varieties in different seasons,the F enrichment tea varieties number in three different degrees were 4 for high,14 for medium and 37 for low,while three levels of Al enrichment were 4 for high,22 for medium and 29 for low respectively,The number of three enrichment levels of REE were1 for high,19 for medium,35 for low respectively,and different habitats and drawed a significant correlation between REE,F and Al in the tea plant when the they are absorbed,we also identified some common low-enriched absorption of F,Al varieties such as 'T13','T14','Xiinrencha' and so on.Thus providing reference for the next screening of anti-aluminum and anti-fluoride tea cultivars and gives a reference for the production of planting material when picking and processing tea fresh leaves.(2)To analyze the content of REE,F and Al elements between the different tea mowing height,After mowing,the 3 kinds of tea plant heights each are 20cm,40cm and 60cm,measured their REE,F,Al element content,the study showed an increases with tea plant height,REE and Al content was gradually decreased,mowing height 20cm(0.36mg/kg)has the max REE content which was significantly higher than traditional canopy height 60cm(0.12mg/kg),while the tea plant had the max F content at mowing height 40cm;amino acid content of tea can be an appropriate increase when mowing height higher and got a favorable quality,total catechins and polyphenols have different performances,with the height decreases,they performed increasing products of REE,F and Al content in fresh leaves roughly trimmed qualitative agreement.The REE,F content between the Al content was significantly correlated,REE,Al content also showed a significant positive correlation with tea catechins.The study provides the basis for clarifying the relation of tea plant height and REE,F and Al content and solve the accumulation characteristics problem in tea plant.(3)After sequencing of 3 different tea varieties(lines)-T15,Benshan and Shuixian using RNA-seq technology,We obtained 16.44 Gb data.After de novo assembly and filtering the original data,we finally received a total of 83357 UnigenesThe biological information of the tea Unigenes that annotated to 8 database:ALL-Unigene,NR,NT,SwissProt,KEGG,COG,GO and Pfam.there were 36118,30742,22870,19896,12302,28024 annotation results respectively,total got 40707 Unigenes.Through the analysis of GO,COG and the KEGG pathways of comments by the success annotated UniGenes,through the GO function classification,in the tea Unigenes,18967 differences were classified into 44 functional categories;differentially expressed genes involved in COG classification results are mainly classified into general function prediction of signaling pathways and transcription pathway;of all the tea Unigenes there were 4775 successful annotation to 201 signal pathway.Among them,3 samples the most significant enrichment,of those the most critical was the metabolic pathways in plant hormone signal transduction pathway.(4)In order to identify the accuracy of RNA-seq technology data and find the key genes which caused different accumulation of REE,F,Al element,the qRT-PCR technique was used to find key genes in 3 different tea varieties(lines),the results showed that the RNA-seq technology data is true and reliable.In addition,we also collected 6 differential expression genes from 3 tea varieties(lines),expanded to 10 tea varieties(lines)for further verification and found that the relative expression genes in 10 different tea varieties(lines)reasearch a highly differential level Two gene fragments related to REE content were found through correlation analysis,they are,MTP andbZIP transcription factors(r=0.636 and 0.652),both reached a significant level;While 1 gene fragments related with F content was MTP(r=0.675);Two gene fragments related with Al content were ABC transporter and bZIP transcription factors(r=0.872 and 0.706).The ABC transporter gene and Al content reached an extremely significant positive correlation.ABC transporter might participates in the positively regulation of metal elements absorption and accumulation behavior. |
PDF Full Text Request