Difference In Spore Developmental Morphology And Ecp Effectors Of Two Formae Specials Of Marssonina Brunnea

Two formae specials of Marssonina brunnea in Populus genus cannot be distinguished easily based on their morphologies and some nucleic acid molecular sequence marks.Researching the difference of microspores process,applicating new technology to provide a new direction for identification of the pathogen and its interactions with host relationship research.This study using collection from different sources of pathogenic fungi to total the PCR amplification of DNA as a template for the ITS sequence,joining the construction of phylogenetic trees and ITS2 secondary structure to differentiate between the two type specialization.Using fluorescent whitening agent to observe spores under the condition of different development process.Building two formae specials strains with affinity for the host system.Determination of three spores of the two formae specials strains during the development of secreting proteins.The results show that:(1)Determining the colony and basic morphometry of two formae specials strains,founding that different sources of differeng formae specials strains have no obvious difference.The way to build ITS sequence phyligenetic tree just can explain the relationship of marssonina's interspecies,it cannot reference ITS sequence accurate positioning in the specialized type.The formae specials strain's structural model in ITS2 secondary structure is same,but there are multiple sites base differences.Using ITS2 secondary structure joint systems development tree can be more effective to distinguish the formae specials of marssonina brunnea.(2)By measuring 5 strains of two formae specials,repeating for 50 spores of each strain,statistical analysis,founding that the single germ tube compared with more germ tubes are more length and narrow,but is not obvious.Two formae specials strains' spores can all complete the whole development process in water;On the cellophane also can complete the whole process of development the same as in water.(3)Through the inoculation experiment,establishes the strains with affinity for the host system.Using For fluorescence microscope to observe pathogen infection process,discovering that between the compatibility and non-compatibility host,spore development process has obvious difference.On the compatibility host,it can be observed the entire development process.In the non-compatibility host,the development process is not complete.After developing to the stage of appressorium,the development is stop.Some continuously produced appressorium,until stop growing.(4)Determining the two formae specials each three fungus spores'development secretory protein on the cellophane,taking an analysis of spores in the whole development process of the difference of secretory proteins.By the analysis of the measuring protein contains 20 Ecp family proteins,eight of them have been previous validation for the effect of protein,concluded that the effect of protein 12 for speculation.