Prescription Screening,Safety And Clinical Efficacy Of Eupatorium Lindleyanum Powder

Avian infectious bronchitis(IB)is an acute case of coronavirus infectious bronchitis virus,highly contagious respiratory disease.It is characterized by sudden gasp,tracheal rales,coughing,sneezing,runny nose,swollen eyes and other symptoms.The mortality rate reaches 10%to 40%and laying rate declined sharply.Poultry respiratory disease in poultry respiratory infections mainly refers to disorders,such as poultry,chronic respiratory disease,infectious bronchitis,infectious laryngotracheitis,aspergillosis,etc.These common,multiple infectious diseases,serious impact on the poultry growth and development,as well as causing a large number of deaths caused huge losses to the poultry production.West Veterinary multi-use prevention and treatment of these diseases antibiotics and chemicals,as after a long unreasonable application,in addition to drug toxicity is likely to cause greater than poisoning,but also lead to drug resistance and drug residues in egg products and endanger human health.Application of Chinese medicine prevention can overcome these drawbacks.The study screened out based medicine to chase Mustang Mustang chase clenbuterol scattered,and studied the safety and clinical efficacy of the party for chickens,aims to develop better prevention and treatment of respiratory disease in poultry medicine preparations.The research is divided into the following sections.Test 1.The prescription screening of eupatorium lindleyanum powder 42010-day-old,"288" chickensu was infected useing nasal drip IBV and Haemophilus paragallinarum equivalent mixture of ways.The Haemophilus paragallinarum chicken soup chicken serum were seeded in medium containing 2%hemoglobin in sheep,37 ? cultured 24h,100-fold dilution,were seeded in 10-day-old chick embryos,each embryo allantois 0.1 mL,37 ? incubator 48h,take allantoic liquid reserve and observe the embryo lesions.To be elected for three Chinese herbal compound screening tests.The results showed that three of the chicken respiratory compound has a therapeutic effect,but one of the most obvious effect of compound A,its treatment efficiency is significantly higher than the other two compound can be selected compound A(Bronco chase 40g,20g skullcap forsythia 15 g,bellflower 15g,licorice 10g)as a drug product development.Test 2.The acute toxicity assay of of eupatorium lindleyanum powder Mustang chase clenbuterol acute toxicity,according to the "Veterinary test specification compilation" requirements,using the modified Karber method,use mice as experimental animals to cook crude drug effective ingredient extracted using gavage acute toxicity tests.The results showed that the Broncos chasing clenbuterol bulk water decoction of mice LD50 of 330.94 mg(crude drug)/g(body weight),95%may be limited to 281.96?409.22 mg(crude drug)/g(body weight),according to the "Veterinary test compilation of technical specifications,"the evaluation criteria should be sentenced to actual non-toxic.Test 3.The subchronic toxicity assay of eupatorium lindleyanum powder In order to study the Broncos chase clenbuterol scattered in the course of security,carried out on rats sub-chronic toxicity tests.The 100 SD strain rats were randomly divided into four groups,each group 25 with half of male and female.Set a negative control group and three dose groups gradient(10,100,300 mg/g diet).Test period is 90 days.The results showed that clenbuterol Mustang chasing loose no adverse effects on the body of each experimental group of animals,weight gain,organ coefficient for each test group animals were similar to the control group,blood count,blood chemistry test group of animals,some indicators and the control group had no significant difference(P>0.05),visual observation of the results of each group organs no obvious abnormalities.The results showed that the Broncos compatibility chase after clenbuterol scattered toxic side effects.Test 4.The clinical therapeutic effect observation of eupatorium lindleyanum powder for gallinaceous infectious bronchitis 200 Roman Roosters diagnosed as infectious bronchitis were randomly divided into four groups,each 50.Bronco chase clenbuterol scattered high,medium and low-dose group were chasing each feather fed clenbuterol Mustang Powder 1.5 g,1.0 g and 0.5 g,drug control group were fed every feather slow call San 0.5 g,2 times a day for 6 days.In the first two days after stopping the cure rate and survival statistics for each group of chickens infected and average weight change,integrated to determine efficacy.The results showed that the Broncos chasing clenbuterol scattered high cure rate dose groups were 82%,80%,significantly higher than the control group of drugs(54%)and low-dose drug group;after treatment,high dose group of infected was significantly lower than the average weight gain was significantly higher than the low-dose group and the drug and drug control group,no significant difference between the dose groups.Bronco chase showed gram powder on avian infectious bronchitis asthma have a better therapeutic effect,significantly better than the slow call casual recommended dose(chicken every feather each 1 g)dose for clinical application.