Study On Inhibition Of Plant Extracts On Botrytis Cinerea

Tomato gray mold is a global disease caused by Botrytis cinerea.This disease,which can cause decrease of production up to 50%,can infect many kinds of horticultural plants,such as tomatoes,cucumbers and eggplants.It can lead to great economic damages.Chemical microbicide is the most common treatment to protect the plants.However,the gene diversity is extremely high in the communities of this mold because of heterocaryosis,which makes this mold more likely to generate drug resistance.Hence,chemical microbicide cannot solve this issue properly while giving rise to a series of environment problems.Many plants,especially medicinal plants have a powerful ability to resist microorganism.This study aimed to establish a rapid and efficient system of medicinal plant selection,which can screen out plant materials containing compound against Botrytis cinerea from a large number of plants to solve this issue.The selection system can conduct the antimicrobial activity test on several plants extracts in one plate through Paper Disk method,separate and purify antimicrobial compounds as well as evaluate its antimicrobial ability by plate mycelial growth rate method,which is more accurate(add the extracts directly to the plate.We have selected a plant(Uncaria sessilifructus)with high anti-Botrytis cinerea activity,from 100 kinds of major medical species in Yunnan province,China.Major antimicrobial compound A(EC50=240.42 ?g/mL)and its synergy compound B were isolated from this species.In conclusion,We found out a potential plant microbicide against Botrytis cinerea,which can be a good material for inhibition mechanism research,and proved the practicability of this selection system.