Investgation Of Major Respiratory Diseases Of Bovine And Sheep In Guangxi

In order to master the infection situation of the respiratory disease of bovine and sheep in Guangxi.In this study,we investigate the serum and pathogenic factors,and the result as follows.(1)In 2016-2017,bovine and sheep serum from Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin were tested for the antibody of Bovine rhinotracheitis,Mycoplasma bovis and Mycoplasma ovipneumoniae,each animal has 2,400 serums.Bovine rhinotracheitis antibody testing results show that in 2016,Guangxi Bovine rhinotracheitis antibody individual positive rate is 27.33%(328/1200),group positive rate is 58.93%(33/56),among them,Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin Bovine rhinotracheitis antibody individual positive rate is 17.5%(35/200),22.5%(45/200),51%(102/200),34%(68/200),22%(44/200),17%(34/200),group positive rate was 42.9%(3/7),71.4%(5/7),80%(8/10),50%(5/10),62.5%(10/16),33.3%(2/6);And in 2017 Guangxi Bovine rhinotracheitis antibody individual positive rate is 45.58%(547/1200),group positive rate is 81.82%(63/77),among them,Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin individual positive rate of Bovine rhinotracheitis antibody is 15.5%(31/200),53.5%(107/200),81%(162/200),43%(86/200),48.5%(97/200),32%(64/200),group positive rate of which is 58.3%(7/12),100%(6/6),95%(14/15),80.3%(12/15)and 82.35%(14/17),85.7%(6/7).Compared with 2016,the infection of Bovine rhinotracheitis in Guangxi is on the rise.Mycoplasma bovis antibody test results show that in 2016,Guangxi Mycoplasma bovis antibody individual positive rate is 23.33%(280/1200),group positive rate is 59.2%(36/61),among them,Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin individual positive rate of Mycoplasma bovis antibody is 28%(56/200),48.5%(97/200),20%(40/200),2%(4/200),27%(54/200),14.5%(29/200),group positive rate of which is 66.6%(8/12),71.4%(5/7),80%(8/10),30%(3/10),56.25%(9/16)and 50%(3/6);In 2017,Guangxi,individual positive rate of Mycoplasma bovis antibody is 38.5%(462/1200),group positive rate is 74.62%(50/67),among them,Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin individual positive rate of Mycoplasma bovis is 46.5%(93/200),54.5%(109/200),46%(92/200),29%(58/200),34%(68/200),21%(42/200),group positive rate is 85%(6/7),100%(6/6),93%(14/15),60%(9/15),58.8%(10/17),62.8%(5/7).Compared with 2016,the infection rate of Mycoplasma bovis is increasing in Guangxi.The infection rate of Bovine rhinotracheitis and Mycoplasma bovis is 12.21%(293/2400).Double infection,the ratio of the number of infections are 33.48%(293/875)and 39.48%(293/742),secondary infection of cattle infected with Bovine rhinotracheitis disease probability is greater than the infection of Mycoplasma bovis secondary infection probability of Bovine rhinotracheitis disease.Mycoplasma ovipneumoniae antibody detection,according to the results of Guangxi in 2016 individual positive rate of Mycoplasma ovipneumoniae antibody is 32.2%(386/1200),the group positive rate of which is 72.88%(43/59),among them,Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin Mycoplasma ovipneumoniae antibody individual positive rate is 47%(94/200),28.5%(57/200),27%(54/200),16%(32/200),36%(72/200),38.5%(77/200),group positive rate is 75%(6/8),62.5%(7/8),78.57%(11/14),63.6%(7/11),87.5%(7/8),50%(5/10);And 2017 in Guangxi Mycoplasma ovipneumoniae antibody of individual positive rate is 49.2%(590/1200),group positive rate is 82.61%(38/46),among them Guigang,Hezhou,Baise,Liuzhou,Nanning,Laibin Mycoplasma ovipneumoniae antibody individual positive rate is 78%(156/200),54%(108/200),38%(76/200),33%(66/200),43%(86/200),48%(96/200),group positive rate is 84.61%(11/13),83.33%(5/6),83.33%(5/6),66.6%(4/6),87.5%(7/8),85.7%(6/7).Compared with 2016,the infection rate of Mycoplasma ovipneumoniae in Guangxi increased significantly.(2)By PCR/RT-PCR method,bacteria separation and identification in 2015-2017,63 bovine respiratory symptoms diseases and 73 sheep respiratory symptoms diseases been tested,and 7 Mycoplasma bovis have been isolated in pathogenicity test and drug susceptibility test.The main pathogenic factors of the respiratory symptoms of bovine and sheep were Mycoplasma bovis 30.86%(25/81),with multiple infection 28.57%(18/63),and Mycoplasma ovipneumoniae 49.63%(68/137),with a multiple infection 73.97%(54/73).The Mannheimia haemolytica detection of bovine and sheep was 12.84%(28/218).In addition,many disease been detected in bovine,Mannheimia haemolytica 13.58%(11/81),Klebsiella pneumoniae 11.11%(9/81),and Arcanobacterium pyogenes 8.64%(7/81).Many disease been detected in sheep,Mannheimia haemolytica 12.41%(17/137),Klebsiella pneumoniae 5.84%(8/137),and Arcanobacterium pyogenes 5.84%(8/137).Among the 7 Mycoplasma bovis,the strongest pathogenic force is Guangxi Shanglin YS and the weakest pathogenic force is Guangxi Guilin gl 2.The drug sensitivity tests show that 7 Mycoplasma bovis are sensitive to fluoroquinolone and macrolides,resistant toβ-lactam,intermediary to tetracycline and aminoglycoside.(3)PCR,cloning and sequence analysis are carried out on the cloned surface membrane protein P81 gene and the surface variable lipoprotein vspY1 gene.Sequence analysis show that the P81 gene of 7 Mycoplasma bovis is more conservative and less mutated.And the vspYl gene variant is large.The results of online antigenic analysis show that the variable main antigen gene loci of vspYl is 241-266,which amino acid sequence is"ELQKKYASLLPIVTEFFKLHEKIVSK".(4)With Hemolysis mansteria’s sialoglycoprotease(Gcp)gene of target genes,design of ring mediated method of isothermal amplification(LAMP)detection primer,the LAMP method is high specificity,as sensitivity as PCR,in 25 minutes to complete the testing,shorten the 3.5 times compare to PCR.The application of LAMP and PCR are used to detect 10 cases of Hemolysis mansteria,the coincidence rate of 10 cases with common PCR was 100%.Conclusion:(1)the individual positive rates of infectious Bovine rhinotrachetis,Mycoplasma bovis and Mycoplasma ovipneumoniae in 6 cities of Guangxi from 2016 to 2017 are all higher than that of 2016.(2)2015-2017,the main respiratory pathogens of bovine and sheep in Guangxi are Mycoplasma bovis and Mycoplasma ovipneumoniae.Mannheimia haemolytica is a potential threat to the respiratory diseases of bovine and sheep.The pathogenic of Guangxi Shanglin YS is the strongest,Guangxi Guilin gl2 is the weakest in Guangxi.The preferred drug of Guangxi Mycoplasma bovis is fluoroquinolone,which can be used in combination with macrolide.(3)the P81 is stable in Guangxi,and there is large variation in the vspYl gene.7 Mycoplasma bovis in Guangxi vspY1 common sites of antigens on the surface of the amino acid sequence of"ELQKKYASLLPIVTEFFKLHEKIVSK".(4)the LAMP method constructed in this study has been used to detect the specific high specificity of Hemolysis mansteria,which can be rapidly used for Hemolysis diagnosis.