Whole Genome Mining Of Tomato BES1 Transcription Factor And Screening Of Stress Resistance Related Genes

BRI1-EMS-suppressor 1(BES1)is a transcription factor(TF)that functions as a master regulator of brassinosteroid(BR)-regulated gene expression.As a plant-specific transcription factor,BES1 not only plays an important role in plant growth and development,but also is closely related to disease resistance,hormonal response,biological and abiotic stress.At present,there are many reports on the BES1 transcription factor family,such as Arabidopsis thaliana,Chinese cabbage,Brassica napus,and cotton,but the study of tomato BES1 has not been reported.In this study,we performed a systematic overview of the systematic excavation and bioinformatics analysis of the tomato(Solanum lycopersicum)BES1 gene,performed bioinformatics analysis,and further analyzed the expression pattern of the tomato BES1 transcription factor.Genes were screened and identified,and functional verification was performed on genes with significant resistance responses.The aim is to provide a theoretical basis for related research on tomato stress-resistance genes.The test results are as follows:1 Through bioinformatics analysis,nine tomato BES1 transcription factor family members were screened.Grouped by cluster analysis,all the above genes were divided into three subfamilies.The BES1 transcription factor family is located on the 8 chromosomes of tomato.Although different plants have their specific functions,bioinformatics analysis shows that the BES1 gene has similarities to other plant proteins.2 The expression pattern of BES1 gene in different tissues was analyzed by fluorescence quantitative PCR.The results showed that the expression of each gene in different tissues was different,and the same gene was expressed in different tissues.Nine genes were highly expressed in roots,stems,leaves,and flowers,and were lower in fruits.The SLB6 gene has a high expression level in roots and stems and a low expression level in fruits.3 The study found that BES1 gene was induced by a variety of stress factors under abiotic stress,especially under salt stress,the expression of all genes showed a significant increase.Under drought stress,SLB3,SLB4,SLB7 and SLB9 showed a significant decrease and then increased.Under cold stress,the SLB2 gene and SLB9 gene showed a clear increase and then decreased.4 The two genes used for identifying the functions were SLB3 and SLB9.The gene silencing vector was successfully constructed.Using Agrobacterium-mediated method,the corresponding gene silencing plants were cultivated,and the tomato gene silencing plants were treated with drought and salt stress.Combined with the results of fluorescence quantitative PCR and physiological indicators,it can be seen that SLB3 gene and SLB9 gene are related to the mechanism of plant stress resistance regulation,and may play a very important role.