Bioinformatic Analysis And Identification Of Some Resistance-associated Genes Of GRAS Gene Family In Tomato

The GRAS gene family is a plant-specific gene family,which had been found to participate in the complicated biological process in many plants,such as herbaceous plants,woody plants and aquatic plants.Especially,the close relation between GRAS genes and the response of adversity stress.The research of the GRAS gene family about structure prediction and function analysis in the model plant Arabidopsis thaliana were more profound and comprehensive,using bioinformatics methods and software to identify the members of the GRAS gene family in tomato genome.At the same time,expression analysis with some GRAS genes was performed to characterize GRAS gene family systematacially,providing an experimental basis for further functional study of GRAS transcription factors in tomato.The results showed that:1.53 GRAS genes were identified and located on 12 tomato chromosomes,Phylogenetic results showed that those genes could be classified into ten groups,they are ?,?,?,?,?,?,? and ?.The analysis of chromosome localization showed that the GRAS genes present uneven distribution on 12 chromosomes.Not all the GRAS genes were highly conserved in tomato.Conserved motifs analysis indicated that a part of GRAS gene family was highly conserved.2.Expression pattern analysis of some typical GRAS genes showed that different tissues and organs of the tomato relative expression is higher in root,relative expression in the stem and fruit is low by comparing to express content in leaf.3.The osmotic stress response analysis showed that,those 12 GRAS genes expression were induced by the stress to increase at different degrees.4.Building pTRV-SLGRAS43 vector and transducing to tomato seedlings successful,and the result showed that SLGRAS43 gene participated in the response process of drought and cold.