Epidemiologic Study And Zoonotic Risk Analyze In Intestinal Parasites In Hebei And Tianjin

With the continuous development of dairy cattle breeding,parasitic diseases have become one of the most common diseases in dairy cattle breeding.In order to understand the prevalence of intestinal parasites in dairy cattle in Hebei and Tianjin,near to Bohai area,the centrifugal precipitation,Sheather's sugar flotation,and Lugol's iodine staining techniques were used to identify 1040 fecal samples from12 dairy cattle farms in Hebei and Tianjin areas.The overall infection rate was61.63%,with the prevalence of 59.78% and 66.12% in Hebei and Tianjin,respectively.Eight parasites species were identified including Coccidium spp.,Giardia duodenalis,Cryptosporidium spp.,amoeba,Strongylus spp.,Trichuris spp.,cestode,and trematode,and the infection rates were 40.96%,1.63%,8.46%,33.27%,2.02%,3.56%,0.96% and 0.96%,respectively.Coccidia spp.and amoeba were the predominant parasites.A total of 1040 fecal samples were screened for Cryptosporidium spp.,Giardia duodenalis and Enterocytozoon bieneusi by polymerase chain reaction.The overall prevalence for Cryptosporidium,G.duodenalis and E.bieneusi was 1.0%(n=10),4.7%(n=49)and 19.4%(n=202),respectively.Ten Cryptosporidium-positive samples were identified as C.parvum by DNA sequence analysis of the small subunit r RNA(SSU r RNA)gene.DNA sequencing of the 60-k Da glycoprotein gene revealed that the C.parvum samples were all subtype IId A19G1.Forty-nine G.duodenalis-positive samples belonged to assemblage E(n=47)and assemblage E mixed with A(n=2),based on the sequenced SSU r RNA,triosephosphate isomerase,and glutamate dehydrogenase genes.Sequence analysis of the internal transcribed spacer(ITS)gene identified six known E.bieneusi genotypes,I(n=87),J(n=83),BEB4(n=18),BEB6(n=3),N(n=1)and Ebp C(n=1),along with three new genotypes,CHC6(n=1),CHC7(n=1)and CHC8(n=7).Phylogenetic analysis showed that Ebp C belonged to zoonotic group 1,whereas the other E.bieneusi genotypes clustered within group 2.More studies are needed to better understand the species distributions and public health significance of these pathogens in the study areas.