Diversity Of Bacteria And Phosphate Solubilizing Bacteria In Rhizosphere Soil Of Phyllostachys Edulis Based On Phosphorus

Phyllostachys edulis,which has the characteristics of fast growth,early timber and high economic value,is one of the most important forest resources in the country.It is also one of the most important resources for the processing and utilization of Phyllostachys edulis products in Jiangxi,and is one of the main ways to increase the income of farmers.Phosphorus is one of the essential nutrients for the growth of Phyllostachys edulis.The phosphorus deficiency in the red soil region of the south of China is prominent,and most of the phosphorus in the soil is combined with calcium,iron and aluminum plasma to lose its effectiveness,and it is difficult to be absorbed directly by Phyllostachys edulis.Therefore,it is very important for the Phyllostachys edulis to absorb and make full use of phosphorus to transform the phosphorus which is difficult to dissolve to the dissolved phosphorus.In this study,the rhizosphere soil of Phyllostachys edulis was studied under different phosphorus conditions,namely,high phosphorus addition(50kg hm^-22 yr^-1),low phosphorus addition(25kg hm^-22 yr^-1)and CK(0kg hm^-22 yr^-1).The combination of traditional soil microbial culture and advanced high-throughput sequencing method was used to study different phosphorus addition strips.The species and distribution characteristics of phosphate solubilizing bacteria in rhizosphere soil of Phyllostachys edulis,the diversity of bacteria population in rhizosphere soil of Phyllostachys edulis under the conditions of different phosphorus addition and the interaction mechanism between the soil chemical properties and the soil chemical properties;the main results are as follows:1)In this experiment,a total of 28 phosphate solubilizing bacteria were isolated from the rhizosphere soil of Phyllostachys edulis forest in Shuang Zhen forest farm,Guixi,Jiangxi province.The pH value of each strain had a significant negative correlation with phosphate solubilizing capacity of phosphate solubilizing bacteria,which is consistent with many research results.The phosphate solubilizing capacity of 28 phosphate solubilizing bacteria screened was between 42.92mg/L and 79.37mg/L.The highest phosphate solubilizing capacity of strain XQP33 was 79.37mg/L,Enterobacter.Through identification and sequence alignment,the results showed that 7 strains were similar to Burkholderia,12 strains,Enterobacter,3 strains,Klebsiella,6 and Raoultella sp..From the result of diversity index,it can be concluded that the diversity of phosphate solubilizing bacteria increased in the plots where phosphorus was added.However,the phosphorus content had little effect on the diversity of phosphate solubilizing bacteria,and the diversity index of phosphate solubilizing bacteria was higher than that of high phosphorus.It indicates that low phosphorus addition can promote the growth and development of phosphate solubilizing bacteria.Compared with different altitudes,the diversity of phosphate solubilizing bacteria in rhizosphere of Phyllostachys edulis was higher than that in 300m area at 800m altitude.The difference of altitude also affects the population diversity of phosphate solubilizing bacteria.After the application of phosphate fertilizer,the diversity of phosphate solubilizing bacteria community was increased.The results showed that the number,diversity and richness of phosphate solubilizing bacteria under phosphorus addition were greater than those in control plots.It can be seen that after the addition of phosphorus,the population diversity of phosphate solubilizing bacteria in rhizosphere soil increased,and the growth of phosphate solubilizing bacteria was promoted.2)Through high throughput sequencing,we can see that the bacteria in this study area have 49 gates,94 classes,171 orders,578 genera,and the dominant bacteria gate Proteobacteria,Acidobacteria,Nitrospirae,Actinobacteria,Verrucomicrobia,Planctomycetes,Firmicutes,Chloroflexi,Gemmatimonadetes,Bacteroidetes,etc.The class of bacteria is Alphaproteobacteria,unidentified_Acidobacteria,Betaproteobacteria,Clostridia,Planctomycetacia,Gammaproteobacteria,Spartobacteria,Deltaproteobacteria,Thermoleophilia,Ktedonobacteria,Nitrospira,etc.Acteriales,Burkholderiales,Clostridiales,Planctomycetales,Frankiales,Chthoniobacterales,Subgroup₃,etc.;the dominantbacteriabelongtoAcidothermus,Bradyrhizobium,Massilia,Candidatus_Solibacter,Variovorax,declining,restricting,etc..The above results indicate that there are abundant species of bacteria in rhizosphere soil of Phyllostachys edulis.3)This study found that the effects of Different Phosphorus Additions on soil nutrient content in rhizosphere soil of Phyllostachys edulis were different.The effects of phosphorus addition on rhizosphere soil nutrients?except effective phosphorus content?of Phyllostachys pubescens were significantly different.4)The relative abundance of microbial taxonomic groups is similar to that of organic matter under different organic matter contents.For example,most of the phylum abundance was related to organic matter.The content of organic matter in rhizosphere soil of Phyllostachys edulis was negatively correlated with Thermotogae and was significantly negatively correlated with MCG and Thaumarchaeota,but the organic matter content of rhizosphere soil was positively correlated with Actinobacteria and was significantly positively correlated with Planctomycetes.In different phosphorus treatments,the relative abundance of Proteobacteria was high in phosphorus treatment,which may be related to soil organic matter and pH.