| With the development of the large-scale pig industry,early weaning has been widely adopted in pig production.Due to the physiological characteristics of weaned piglets,the efficiency of energy utilization is particularly critical.Therefore,improving the efficiency of energy utilization in weaned piglets is an important way to increase the performance of weaned piglets and pig production efficiency.Guanidinoacetic acid is ubiquitous in animals and is a precursor for the synthesis of creatine and is therefore closely related to the body's energy use.In order to study the effects of Guanidinoacetic acid supplementation on the performance of weaned pigs and their mechanisms,we used feed and slaughter test methods to observe the effect of Guanidinoacetic acid on the performance of weaned piglets.Simultaneously,the serum and intestinal tract of piglets were tested.Changes in barriers,protein deposition,and markers related to creatine metabolism,and to explore the mechanism of Guanidinoacetic acid on energy metabolism in weaned piglets,provide experimental evidence for the popularization of Guanidinoacetic acid.In this study,204 healthy 28-day-old weaned piglets(Duroc × Landrace × Yorkshire)were randomly divided into three groups.Group A was the control group and was fed the basal diet.Group B was the animal protein-free group.The basal diet without animal protein was used;the C group was a Guanidinoacetic acid group and was fed a diet supplemented with 600 ppm Guanidinoacetic acid in the animal protein-free diet.Each group had 4 replicates and each replicated 17 pigs.Four weeks of the trial,free intake of food and drinking water and record daily feed intake to observe the health status of piglets.At the end of the test,8 piglets weighing approximately the average weight were selected for slaughter sampling.The relevant physiological and biochemical indicators,intestinalbarrier function-related indicators,mRNA expression of muscle protein deposition-related factors,and creatine metabolism-related enzyme activities in vivo were determined.Related gene mRNA expression levels.The research results are showed as follows:1.Addition of 600 ppm Guanidinoacetic acid to diets can significantly increase the average body weight gain of weaned piglets,significantly reduce feedweight gain ratio of weaned piglets,and increase the performance of weaned piglets.2.Adding 600 ppm Guanidinoacetic acid to the diet can significantly reduce the serum endotoxin content of piglets,significantly increase the expression of Occludin and Claudin-1 protein in the duodenum and the expression of Occludin protein in the jejunum(P ? 0.05).The mRNA expression of anti-inflammatory cytokine IL-10(P ? 0.05)significantly increased the number of goblet cells in the jejunum and ileum of weaned piglets(P ? 0.05),and decreased the total bacterial count in the ileum of weaned piglets(P=0.076).)shows that Guanidinoacetic acid can improve intestinal barrier function.3.The addition of 600 ppm Guanidinoacetic acid to the diet had no effect on mRNA expression of protein related genes of longissimus muscle in weaned piglets and creatine metabolism in kidney,liver and longissimus dorsi muscle.4.The addition of 600 ppm Guanidinoacetic acid to the diet had no significant effect on the endogenously synthesized Guanidinoacetic acid in weaned piglets,but it could significantly reduce the increase in the synthesis of endogenous Guanidinoacetic acid due to the absence of animal protein feeds.Increase,reduce the consumption of arginine in the body;significantly increase the GAMT enzyme activity in the kidneys of weaned piglets,and promote the synthesis of creatine.In summary,the addition of 600 ppm Guanidinoacetic acid to the diets of weanling piglets can promote the growth of piglets and improve the efficiency of feed utilization.The addition of Guanidinoacetic acid to diets has no significant effect on the development of intestinal villi in piglets,but it can increase intestinal growth.Barrier function. |
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