| Heat stress caused huge lose to animal production,especially to hens industry.There’re several factors mainly include environment,feed and strain that stress poultry most.And all kinds of stress from outside environment cause oxidant stress.Recent reports showed that the strength of oxidation resistance and the balancing status of oxidation and antioxidation were the health indicators for hens.This study was conducted based on the rich learning experience of anti-stress feed additives in our laboratory.And it’s the first time to take ginger powder into this field to investigate the anti-heat stress and antioxidant effect of ginger powder,Chinese herbal medicine and their mixture,the curative effect of ginger powder,Chinese herbal medicine and their mixture to the body antioxidation and organ injuries of hens in aspects such as metabolism,and the effect of ginger powder,Chinese herbal medicine and their mixture to oxidation resistance of hens with heat stress in system levels of organism,tissue,cell and protein.This study mainly include:EXP 1.Effects of ginger powder,Chinese herbal medicine and their mixture on laying hens at HATsTwo hundred and twenty 25-week-old lohmann laying hens with similar weight and health condition were randomly divided into 5 groups(NC / HC / H1 / H2 / H3)with 4 replicates each and 11 hens in each replicate.The performance,serum metabolism indexes and antioxidant activity of hens were determined and analysised.It’s found that ginger powder,Chinese herbal medicine and their mixture all could improve the performance and antioxidant level of hens at HATs.The badly damage occurred in livers,with ginger powder,Chinese herbal medicine or mixture being improved to the normal level,and it turn to be better with mixture added in the diet of hens at HATs.EXP 2.Effects of different dose ginger powder combined with Chinese herbal medicine on laying hens at HATsTwo hundred and sixty-four 25-week-old lohmann laying hens with similar weight and health condition were randomly divided into 6 groups(NC / HC / 0D / H2 / LD / MD / HD)with 4 replicates each and 11 hens in each replicate.The performance,serum metabolism indexes and antioxidant activity of hens were determined and analysised.The result showed that ginger powder,Chinese herbal medicine and their mixture all could improve the performance and antioxidant lever of hens at HATs.The big damage occurred in livers,with 10 g/kg ginger powder-Chinese herbal medicine mixture being improved to the normal level,and it turn to be better with 10~15 g/kg ginger powder-Chinese herbal medicine mixture added in the diet of hens at HATs.The fitting model result suggested the recommended dose was 11.7 g/kg ginger powder+3.32 g/kg Chinese hearbal medicine.EXP 3.Effects of ginger powder combined with Chinese herbal medicine on function study of heart,liver & kidney of laying hens at HATsThis experiment was designed the same as experiment 2.In this test,we mainly measured and analysised functional enzymes in serum,cell activities and histological structure of heart,liver & kidney of hens.The data suggested that heat stress could decrease the level of functional enzymes of heart,liver & kidney of hens at HATs and the mixture could reduced them,better reduced with 10 g/kg ginger powder-medicine mixture.Besides,heat stress damaged liver > heart > kidney.Additives efficiently improved the cells state and 10~15 g/kg ginger powder-medicine mixture functioned better.Fatty degeneration,granular degeneration,cell swelling,vacuolization were brought to liver by heat stress,while additives could improve the damages,better improved with 10~15 g/kg ginger powder-medicine mixture.In conclusion,heat stress significantly decreased the production,antioxidant ability and damaged main viscera of hens.Supplementations with ginger powder,Chinese herbal medicine and the mixture of them in diet all could release the heat stress on hens in different degrees.However,10~15 g/kg ginger powder-3.32 g/kg medicine mixture got better result and it’s recommended to supplement 11.17 g/kg ginger powder-3.32 g/kg medicine mixture in the basal diet to release heat stress. |