Differentially Expressed MicroRNA Analysis Of Equine Macrophages Infected With Equine Infectious Anemia Virus

In order to study the changes of mi RNA expression in the equine monocyte-derived macrophages?eMDM?infected with equine infectious anemia virus?EIAV?,an EIAV pathogenic strain EIAV_DLV34 was used to infect the eMDMs and the uninfected group as the control.Total RNA was extracted and Illumina HiSeq sequencing was used to analysis the level of mi RNA expression in the cell.The res?lts showed that there are 16differential ression miRNAs compared with the control after infection with EIAV_DLV34.Among them,the expression of eight miRNAs were upreg?lated and the others were decreased.The expression of these 16 miRNAs were verified by quantitative real time PCR,and the res?lts are consistent with Illumina HiSeq sequence analysis.We finally used bioinformatics methods to analysis the target genes and biological processes of these16 miRNAs.Synthetic microRNA mimicsv,we can find that overexpression of mi R-92a can greatly promote the replication of EIAV_DLV34.Furthermore,ananlyzed by luciferase report system,the res?lt showed that miR-92a has no direct effect on virus replcation,but by the indirect effects on the host'target genes to promote viral replication.Synthetic small interfering RNA?siRNA?of the miR-92a target genes CD69,knocking down the expression of CD69 lead to enhancement of the viral replication.Therefore,we have concluded that miR-92a can promote the replication of EIAV_DLV34 by down-reg?lating the expression of membrane protein CD69.This study provides a reference for further analysis of the interaction between EIAV and host,as well as the pathogenesis and the diseases'prevention of lentiviruses.