FMDV Serological Survey Of Luoding City Of Guangdong And VP1 Gene Sequencing Analysis

Foot-and-mouth disease(FMD),caused by foot-and-mouth disease virus(FMDV),is an acute,highly contagious disease of many cloven-hoofed animals.Despite low mortality rates,FMD causes significant economic losses because of the negative effects livestock production and international trade restrictions on animals and animal products.The serotypes O and A of FMDV are major epidemics in C hina so far,the high incidence,complex host range and extensive genetic diversity make the prevention and control of FMD extremely challenging.As the one of the largest provinces of the national pig breeding industry,the breeding industry is important for Guangdong province,so that the prevention and control of FMDV is essential to healthy and stable development of the pig breeding industry.In order to understand the epidemic situation of foot and mouth disease in Luoding C ity,Guangdong Province in recent years,and the immunization protection measures of swine,we tested the FMD-ab of swine serum collected from Chuanbu Pingtang and Sulong in Loding city in Guangdon.And RT-PCR and VP1 gene sequence analysis were performed on pigs with suspected foot-and-mouth disease.Result,the positive rates of FMD-ab type O and A are 94% and 92 % in C huanbu,the positive rates of FMD-ab type O and A are 100% and 98% in Pingtang,the positive rates of FMD-ab type O and A are not more than 50% in Sulong.The analysis of VP1 gene of FMD suspected strains of swine collected from Luoding C ity in Guangdong province,the result and homology analysis showed that the strain had the highest homology with O / BY / CHA / 2010 strain and O / C HA / 31/2010.The isolates may be similar to those of the foot and mouth disease O / BY / C HA / 2010 strain and the O / CHA / 31/2010 strain.This study tests the FMD-ab of swine serum collected from Luoding and identificats of VP1 sequence,and provides the theoretical basis for the prevention and control of epidemic diseases of O-type and A foot-and-mouth disease in Guangdong Province,and provides theoretical guidance for the rational selection of vaccine and disease prevention and control of foot and mouth disease.