Effects Of Environmental Factors On Parthenogenesis Of Laminaria Japonica Female Gametophyte

The female gametophytes isolated and cultured in the laboratory were found to have spores that were easily grown during the preservation of germplasm.In order to better preserve the kelp gamete,more female gametophytes are prevented from being parthenogenesis.In this study,the female gametophyte was induced by the three environmental factors of ferric ion nutrients,temperature and light intensity.The experimental materials were low temperature and weak light(10.0~+1.0?,8?mol/m~2.s)and high temperature and strong light(16.0~+1.0?,100?mol/m~2.s)901,Lianza,and Lianjiang No.1 female gametophyte under two culture conditions.By calculating the ratio of ovulation filaments,ovulation rate,sporophyte incidence to understand what kind of environmental factors easily induce kelp female gametophyte parthenogenesis.1.Effect of iron ion nutrients on parthenogenesis female gametophytesThe iron-free nutrient salt was used as the control group.Three groups were set up for the experiment,and the iron ion concentrations were 0.5,1.0,and 1.5 mg/L,respectively.The culture was observed in a light incubator with a temperature of 10.0+1.0°C,an illumina tion intensity of 20?mol/m~2.s,and a photoperiod of 10:14 h(L:D).Experimental results:The sporulation rate of 901 female gametophyte high temperature and strong light group was higher than that of low temperature and weak light group,and the highest was at iron ion concentration of 1.5 mg/L,which was the highest at 99.17%and 91.13%,respectively;In the strong light group,the sporophyte incidence was the highest at ferric ion concentration of 1.0 mg/L,which was 2.16%and 1.43%,respectively;there was no significant difference between the heterozygous high temperature and strong light group and the low temperature and weak light group,and the iron ion concentration was 0.5 mg.The highest rate of sporophyte formation at0.5mg/L was only 1.27%and 1.5%.2.Effect of temperature on parthenogenesis reproduction of kelp female gametophyteThe experimental set 4 groups,the temperature were 11.0,14.0,17.0,20.0°C,respectively.The culture was observed in a light incubator with a light intensity of 20?mol/m2.s and a photoperiod of 10:14 h(L:D).Experimental results:At a temperature of 20°C,none of the female gametophytes of 901,Lianza,and Lianjiang No.1 had undergone parthenogenesis.At17°C,901,Lianza,and Lianjiang No.1 formation rates were the highest.The sporulation rate of 901 female gametophyte in high temperature and strong light group was higher than that in low temperature and weak light group,which was 16.33%and 77.7%respectively.There was no significant difference between high temperature and strong light group and low temperature and weak light group in plane vegetables,which were 8.67%and 8.63%respectively.The mixed high temperature and strong light group was higher than the low temperature and weak light group,1.7%and 0.93%,respectively.3.Effects of light intensity on parthenogenesis reproduction of kelp female gametophyteThree experimental groups were set up,light intensity was 40,80,120?mol/m2.s,respectively,temperature was 10.0~+1.0?,and photoperiod was 10:14 h(L:D)in a light incubator.Experimental results:Observing the rate of sporophyte formation in 901 female gametophyte.The low rate of sporophyte formation was observed when the light intensity was80?mol/m~2.s and 120?mol/m~2.s,respectively,in the low temperature and weak light group and high temperature and strong light group,which were 88.8%and 87.76%respectively;In Lianza,low temperature and weak light group is higher than the high temperature and strong light group,the highest when the light intensity is 120?mol/m~2.s,respectively 25.8%and20.2%;In Lianjiang No.1,The Low temperature and weak light group and High temperature and weak light groups were the highest when the light intensity was 120?mol/m~2.s and80?mol/m~2.s respectively,which were 26.13%and 5.97%,respectively.The experimental results showed that the female gametophyte 901 is susceptible to parthenogenesis in ferric nutrient,temperature,and light intensity experiments,with the highest ratio of ovulation filaments.The low temperature and weak light groups were 91.07%,38.17%,and 86.33%,respectively,and the high temperature and strong light groups were 100%,63.86%,and 94.66%,respectively.Lianza when the light intensity was 120?mol/m~2.s,female gametophyte was easy to parthenogenesis,with the highest ratio of ovulation filaments,10.17%and 4.63%in the low temperature and weak light group and high temperature and strong light group,and 17°C in the female.Lianjiang No.1 female gametophyte was easy to parthenogenesis at 17°C,with the highest ratio of ovulation filaments,and 8.73%and 12.23%in low temperature,weak light and high temperature light groups,respectively.The ratio of ovulation filaments,ovulation rate,and sporophyte incidence in the low temperature and weak light groups all reached the highest values under the same environmental factors;the ovulation rate and sporophyte body formation rate in the high temperature and strong light groups were basically in the same environment.The factor reached the highest value while the ratio of ovulation filaments differed.