| Wheat is one of the major grain crops in China.Enhancing wheat’s stress-resistance capability is crucial to safeguarding China’s food security.LBD transcription factors play an important role in the regulation of plant growth and stress.In this study,through the rapid development of genomics,transcriptomics and bioinformatics analysis methods in recent years,LBD transcription factors were identified within the whole genome of wheat,and then the physical and chemical properties of the candidate wheat LBD transcription factors were analyzed;Arabidopsis and rice LBD transcription factors and wheat LBD transcription factors were used to construct phylogenetic tree;then the gene structure and conserved domain of wheat LBD gene were analyzed;also the coexpression network of wheat LBD transcription factor based on homologous protein method were constructed;and the expression profiles were explored under the condition of wheat different tissue as well as drought,salt,cold and heat stress,it was found that the highly expressed LBD genes under different tissues and stresses have a very prominent practical significance for enriching the genetic basis of wheat.This study found the following results:1.A total of 75 wheat candidate LBD transcription factors were found through the use of the wheat genome database.According to the results of the alignment and its location on the chromosome,the naming of LBD transcription factors in wheat was named.The analysis of physicochemical properties of candidate wheat LBD transcription factors revealed that the length of wheat LBD transcription factor changes greatly,and subcellular localization finds that most of it is located in the nucleus,and the variation in molecular weight and isoelectric point is relatively large.2.This study construct phylogenetic trees using all LBD transcription factors identified in rice,Arabidopsis,and wheat.The wheat LBD transcription factor family was found to be divided into two categories,Class I and Class II,where the Class I class contained five smaller sub-family of Class IA,Class IB,Class IC,Class ID,and Class IE.In addition,molecular evolution analysis revealed that wheat LBD homologous genes were mainlypurified and selected for evolution.3.The cis-acting element analysis showed that the wheat LBD gene was regulated by multiple growth and stress response elements.It was found that a large number of light-responsive elements were contained in the upstream 1.5k sequence,indicating that the expression of wheat LBD gene may be regulated by light.4.The interaction analysis showed that wheat LBD transcription factors are widely involved in gene interaction.The interaction genes include a large number of growth and stress response genes.Growth and development genes such as IAAs,ERFs,and WOXs,among which IAA is widely involved in auxin-regulated signaling pathways;ERF family members are widely involved in plant growth and development;WOXs participate in cell division and differentiation processes,which in turn regulate plant growth and development;In the regulation of stress,TaLBDs were found to interact with bZIP and MYB genes.Among them,bZIP is a type of transcription factor that is most conserved in plants and plays an important role in plant resistance to abiotic stresses.MYB transcription factors family is a larger type of transcription factor,which plays an important role in plant resistance.It is shown that TaLBDs are widely involved in the growth and development stages of wheat and stress control processes.5.Tissue expression analysis revealed that multiple wheat LBD transcription factors are involved in regulating the development of different tissues in wheat,and have tissue-specific expression.In addition,under the treatment of different stresses,wheat LBD Class I B members were mainly involved in salt stress response,and the expression patterns of Ta-4A-LBD32,Ta-4D-LBD51,and Ta-4D-LBD56 were basically the same.Class II members participate not only in the process of wheat growth but also in stress response. |
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