Effects Of Rumen Protected Pantothenate And Energy Level On Ruminal Fermentation,Nutrient Digestion And Metabolism In Jinnan Steers

In order to evaluate the effects of rumen protected pantothenic acid supplementation on growth performance,ruminal fermentation,digestibility and metabolism and blood indexes of Jinnan steers based on different energy levels diets,the test was divided into two parts and all used 4×4 Latin square designs with four similar average BW Jinnan steers(5 years old).Test I:Control group,LRPP group,MRPP group and HRPP group supplemented with four concentrations(0,0.93,1.40and 1.87 mg/kg BW/d)of rumen protected pantothenic acid.Test 2:Standard energy diet supplemented with 1.40 mg/kg BW/d pantothenic acid(MEP)and protected pantothenic acid in low energy(LERPP),standard energy(MERPP)and high energy(HERPP),respectively.Test 1 showed that average daily gain,digestibilities of OM,DM,ADF,NFE and other relative populations except prevotella ruminicola,rumen a-amylase and protease activities,ruminal effective degradability of DM,OM,ADF in corn silage and OM,DM in concentrate,the concentration of GLU,ACP and CoA enzymes in MERPP group were significantly higher than those of other groups(P<0.05).Nitrogen retained,RN/N,RN/DN,allantoin and total PD in MRPP and HRPP group were dramaticlly increased than those of other two groups.The concentrate of pantothenate and pantothenate kinase in different treatment groups were increased than those of control group,significantly(P<0.05).Xylanase and pectinase activities in MRPP and LRPP groups were higher(P<0.05),while no significant differences between these two groups(P>0.05).Supplemented rumen protected pantothenic acid had no significant effects on pH,mole ratios of acetate,propionate,valerate and concentrate of uric acid(P>0.05),while proportion of acetate and propionate,concentrate of NH3-N were reduced with pantothenic acid amount added.Test 2 showed that in the four groups there were no differences in DM intakes,CP digestibility,concentrates of GLU,SUN,pH,mole ratios of acetate,butyrate,isovalerate and proportion of acetate and propionate(P>0.05).In LERPP group,average daily gain,feed conversion rate,ruminal effective degradability of DM,OM,CP in concentrate and carboxymethyl-cellulase activity were higher than those of other groups,while TG concentrate was lower,significantly(P<0.05).Digestibilities of OM.DM and NFE in MERPP group were increased and digestibilities of ADF,NDF and utyrivibrio fibrisolvens in HERPP groups were also increased(P<0.05).In HERPP group,EE digestibility was increased,however,total VFA,allantoin,total PD,RN/N intake,RN/DN and nitrogen retained were reduced,significantly(P<0.05).In HERPP and MEP groups ruminal effective degradability of DM,OM,ADF and NDF in corn silage had no significant differences,while relative populations of ruminobacter amylophilus and prevotella ruminicola were lower than those of other two groups.In MEP group NH3-N was obviously added.There were no differences of a-amylase and protease activities between LERPP and HERPP group.In conclusion,test 1 showed that optimum addition of rumen protected pantothenic acid was 1.40 mg/kg BW/d.Test 2 indicated that there were interactions between energy and pantothenic acid,low energy diet with optimum rumen protected pantothenic acid could promote the growth and development.While high diet there was no difference on ruminal fermentation but lower DM intakes and nitrogen retained.