| In this paper,the strains of Cordyceps tenuipes C322 and Paecilomyces hepiali C370B2,were used as the object,and the culture medium for raw materials was cultivated by using asparagus.Found that the different periods,different concentrations of variations,eventually the mycelium and to study the changes in chemical composition of culture medium.It was guided the correlation between biomass and chemical composition at each stage of growth.In order to improve production efficiency and provide the scientific basis,Good for selecting high-yield strains,optimize the culture conditions,optimizing the best training time.By selecting the gradient of the culture medium,selecting the 5%concentration of asparagus,Culture and collection were carried out according to the culture time and growth stages.By analyzing the compare in the different growth stages of two kinds of fungi strains and different time periods of culture medium in the process of artificial cultivation variety patterns of the 12 kinds of chemical composition and its distribution patterns of chemical composition of metabolism.The 12 chemical components is polysaccharide,cytosine,total saponins,total flavonoids,uracil,uridine,2'-deoxyuridine,,guanosine,adenine,adenosine,2'-deoxyadenosine and ergosterol.These results were as follows:1 To determine the growth of the two myceliums,Cordyceps tenuipes C322 and Paecilomyces hepiali C370B2,in a medium made only of Asparagus officinalis and pure water.Set the concentration gradient,In the 35 days before the culture of mycelial growth,the biomass showed an obvious trend of increasing.After 35 days,the growth of mycelium biomass began to decline,and it gradually declined,and in 60 days,the powder spores appear;The growth of the biomass in the period from 0 to 45 days of the penicillium was increased.The mycelium began to decline after 45 days,but its biomass was not significantly altered,and the spores in 70 days.In view of that variation trend of mycelium biomass and the different concentration gradient of the culture medium in the course of growth of pseudo trichoderm,the graph is drawn as a correlation between culture time variation,culture medium concentration content and mycelium biomass.And it turns out,In the growth process of mycelium,both of the two species of bacteria have the same biomass as the first increase and then the same.The difference of medium concentration and biomass in the whole growth process was different,and correlation analysis showed that the two were related,and there is a positive correlation between the two before the maximum biomass.Given the correlation between two kinds of fungal mycelium biomass and medium concentration,suggest the best medium concentration is:5%.The best time for harvesting is:C322 should be from 25 to 30 days,and C370B2 be from 35 to 40 days.2.Culture of Cordyceps tenuipes C322 and Paecilomyces hepiali C370B2 with 5%concentration of asparagus,analyze the dynamic changes of the chemical composition of mycelium and asparagus medium at different stages,and draw the following conclusions:for Cordyceps tenuipes C322,The biomass of mycelia reached a maximum of(2.84g)on the 40th day,The biomass of asparagus medium reached its maximum value on the 10th day(4.86g);Take polysaccharides,The mycelium polysaccharide reached its maximum value on the 30th day(3.36mg/g)and asparagus culture medium polysaccharide content reached its maximum value on the 55th day(4.36mg/g).The maximum value of mycelium saponin reached at the 40th day(19.53mg/g),and the maximum value of saponin in asparagus culture medium reached at the 70th day(29.99mg/g).In the case of total flavonoids,mycelium flavonoids reached the maximum value(1.97mg/g)on the 50th day,while the flavonoids content in asparagus culture medium reached the maximum value(7.1mg/g)on the 45th day.Take cytosine for example,mycelium cytosine reached its maximum value(7.313mg/g)on the 30th day,and the cytosine content in asparagus culture medium reached its maximum value(3.424mg/g)on the 40th day.For uracil,the maximum value of mycelial uracil was reached on the 30th day(7.329mg/g),and the maximum value of uracil in asparagus culture medium was reached on the 25th day(3.383mg/g).For uridine,the maximum value of mycelium uridine(7.214mg/g)was reached on the 70th day,and the maximum value of uridine in asparagus culture medium(3.651 mg/g)was reached on the 40th day.In the case of 2 '-deoxyuridine,the maximum value of mycelium 2'-deoxyuridine at the 15th day(20.34mg/g)and the maximum value of 2'-deoxyuridine in asparagus culture medium at the 70th day(5.00mg/g)were obtained.Taking guanosine as an example,the maximum value of mycelium guanosine(74.16mg/g)was reached on the 40th day,and the maximum value of guanosine content in asparagus culture medium(32.04mg/g)was reached on the 10th day.In the case of adenosine,mycelium adenosine reached its maximum value on the 40th day(1179.13 g/g),while the content of adenosine in asparagus culture medium reached its maximum value on the 10th day(649.23 g/g).Taking adenine as an example,mycelium adenine reached its maximum value on the 30th day(2199.7 g/g),and the content of adenine in asparagus culture medium reached its maximum value on the 55th day(3383.80 g/g).In the case of 2 '-deoxyadenosine,the maximum value of mycelium 2'-deoxyadenosine reached on the 25th day(160.66 g/g),and the maximum value of 2 '-deoxyadenosine in asparagus culture medium reached on the 50th day(3.15 g/g).Taking ergosterol as an example,ergosterol content in the mycelium of Cordyceps tenuipes C322 reached its maximum on the 45th day(8987.83 g/g),and ergosterol content in the culture medium of asparagus reached its maximum on the 75th day(1854.00 g/g).The biomass of Paecilomyces hepiali C370B2 mycelium reached the maximum on the 55th day(3.48g),and the biomass of asparagus medium reached the maximum on the 10th day(4.99g).The mycelium polysaccharides reach a maximum of the 40th day,and the asparagus culture is at the maximum in the 10th day of the year,and the sugar is at the maximum(4.99mg/g);Mycelium saponin reached its maximum value(26.55mg/g)on the 45th day,while the saponin content in asparagus culture medium reached its maximum value on the 70th day(36.08mg/g).In the case of total flavonoids,mycelium flavonoids reached the maximum value(2.75mg/g)on the 50th day,while the flavonoids content in asparagus culture medium reached the maximum value(5.75mg/g)on the 50th day.Mycelial cytosine reached its maximum value(6.13mg/g)on day 55,and the cytosine content in asparagus culture medium reached its maximum value(2.677mg/g)on day 40.In the case of uracil,mycelial uracil reached its maximum value(8.63mg/g)on the 35th day,while the content of uracil in the culture medium of asparagus reached its maximum value(5.072mg/g)on the 25th day.For the uridine,the maximum value of mycelium uridine(9.73mg/g)was reached on the 10th day,and the maximum value of uridine in asparagus culture medium(4.272mg/g)was reached on the 35th day.In the case of 2 '-deoxyuridine,the maximum value of mycelium 2'-deoxyuridine at 55 days(10.91mg/g)and the maximum value of 2 '-deoxyuridine in asparagus culture medium at 50 days(5.23mg/g)were obtained.Taking guanosine as an example,the maximum value of mycelium guanosine(49.28mg/g)was reached on the 35th day,and the maximum value of guanosine content in asparagus culture medium(10.29mg/g)was reached on the 10th day.In the case of adenosine,mycelium adenosine reached its maximum value on the 25th day(975.94g/g),while the content of adenosine in asparagus culture medium reached its maximum value on the 50th day(497.82g/g).Taking adenine as an example,mycelium adenine reached its maximum value on the 70th day(1293.3g/g),and the content of adenine in asparagus culture medium reached its maximum value on the 40th day(5222.10g/g).Mycelium 2 '-deoxyadenosine reached its maximum value on the 20th day(53.45g/g).The culture medium of asparagus did not detect 2'-deoxyadenosine content.Mycelium ergosterol reached its maximum value on the 45th day(7547.46g/g),and its asparagus culture medium ergosterol content reached its maximum value on the 15th day(2379.09g/g).3.During the culture period from 0 to 90 days,the contents of flavonoids and cytosine in the culture medium of asparagus were significantly positively correlated with Cordyceps tenuipes C322.The mycelium of Cordyceps tenuipes C322 was negatively correlated with adenosine in asparagus culture medium.The mycelium of Paecilomyces hepiali C370B2 was positively correlated with total flavonoids and cytosine in asparagus culture medium.The mycelium of Paecilomyces hepiali C370B2 was negatively correlated with the content of adenosine in asparagus culture medium.4 The changes of mycelia in PDA medium of Cordyceps tenuipes C322 and Paecilomyces hepiali C370B2 cultured by Asparagus officinalis.To study the chemistry of both the mycelial stages.primary stages and maturation of a sporophore.By analyzing the discovery:For the polysaccharides,C322 primordium(39073.68?g/g)>C370B2 primordium(34268.55?g/g)>C370B2 coremium(29102.52?g/g)>C322 my celium(17998.06?g/g)>C370B2 mycelium(15892.09?g/g)>C322 coremium(14859.59?g/g);For the total saponins,C322 primordium(23818.65?g/g)>C322 mycelium(13267.66?g/g)>C370B2 mycelium(13143.69?/g)>C322 coremium(9125.25?g/g)>C370B2 coremium(8403.75?g/g)>C370B2 primordium(1086.08?g/g);For the total flavonoids,C322 primordium(5589.23?g/g)>C322 coremium(2741.62?g/g)>C322 mycelium(2461.45pg/g)>C370B2 mycelium(1420.89?g/g)>C370B2 coremium(789.88?g/g)>C370B2 primordium(30.91?g/g);For the cytosine,C322 mycelium(966.94?g/g)>C322 primordium(194.66?g/g)>C370B2 mycelium(189.03?g/g)>C322 coremium(120.24?g/g)>C370B2 primordium(79.23?g/g)>C370B2 coremium(68.51?g/g);For the uracil,C322 coremium(165.23?g/g)>C370B2 mycelium(155.85?g/g)>C322 primordium(146.00?g/g)>C322 mycelium(123.72?g/g)>C370B2 primordium(56.9?g/g)>C370B2 coremium(47.86?g/g);For the uridine,C322 primordium(1445.77p.g/g)>C322 coremium(1149.77?g/g)>C322 mycelium(654.13?g/g)>C370B2 mycelium(590.27?g/g)>C370B2 coremium(496.72?g/g)>C370B2 primordium(477.63?g/g);For the 2'-deoxyuridine,C322 mycelium(23.85?g/g)>C370B2 mycelium(8.51?g/g)>C370B2 primordium(6.91?g/g)>C322 coremium(6.84?g/g)>C322 primordium(6.02?g/g)>C370B2 coremium(5.43?g/g);For the guanosine,C322 coremium(1077.73?g/g)>C370B2 mycelium(738.79?g/g)>C322 mycelium(645.43?g/g)>C370B2 coremium(429.14?g/g)>C322 primordium(250.62?g/g)>C370B2 primordium(2.27?g/g);For the adenine,C322 coremium(47.08?g/g)>C370B2 mycelium(42.06?g/g)>C322 mycelium(18.56?g/g)>C322 primordium(4.8?g/g)>C370B2 coremium(4.22?g/g),undetected;For the adenosine,C322 coremium(870.5?g/g)>C370B2 coremium(525.28?g/g)>C322 primordium(454.5?g/g)>C322 mycelium(13.17?g/g),undetected;For the 2'-deoxyadenosine,C322 coremium(49.34?g/g)>C322 mycelium(19.93?g/g)>C370B2 coremium(19.38?g/g)>C370B2 mycelium(7.96?g/g),undetected;For the ergosterol,C322 mycelium(140.83?g/g)>C322 coremium(18.77?g/g)>C370B2 primordium(5.48?g/g),undetected:In this paper,the changes of mycelium and culture medium in different growth stages of cordyceps sinensis strains Cordyceps tenuipes C322 and Paecilomyces hepialid C370B2 were studied using asparagus.It is clear that the two strains of the strain of the species and the chemical distribution of the organisms in different stages of growth,and the selection of asparagus to produce the culture of Cordyceps tenuipes C322 and Paecilomyces hepialid C370B2,Is A reference for that next step of use the asparagus culture Cordyceps tenuipes C322 and Paecilomyces hepialid C370B2 mycelium as ahealth product. |
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