Induction Of Callus In Cerasus Humilis And Optimization Of Adventitious Bud Regeneration System

Chinese dwarf cherry(Cerasus humilis Bge.),also known as calcium fruit,belongs to the Rosaceae family and it is a deciduous shrub fruit tree endemic to China.Because of its rich nutritional value and unique taste,the Chinese dwarf cherry is gradually known to the public.In this study,leaves,embryo,root-like stem from field-grown C.humilis cultivar 'Nongda No.7' and leaves from tissue cultured seedlings of cultivar 'U-1' were used as the materials.Effects of different materials and growth regulators on callus induction,different growth regulators on callus proliferation,different growth regulators on buds induction of root-like stem,and different growth regulators on adventitious buds induction and rooting of C.humilis tissue cultured seedlings were studied.The aim of this study is to build an efficient and stable regeneration system for C.humilis.The main findings are as follows:(1)The results of single factor test showed that MS +2,4-D(0.8 mg L-1)was the most effective for the callus induction of leaves from 'Nongda No.7' with a induction rate of 97.52%,followed by MS + NAA(0.5 mg L-1);Multiple factor test results showed that MS+NAA(0.8 mg L-1)+ 6-BA(1.0 mg L-1)had the 1highest induction rate(98.67%),followed by MS+NAA(0.5 mg L-1)+ TDZ(3.5 mg L-1).The effect of multiple factors on callus induction was higher than that of single factors.(2)The results showed that MS +2,4-D(1.0 mg L-1)was the most effective for the callus induction of embryos from'Nongda No.7'with a induction rate of 93.54%,followed by MS +NAA(0.5 mg L-1).(3)The most suitable single factor for the proliferation of leaf-induced callus was to add 2,4-D(0.8 mg L-1),and the best multiple factor were MS +2,4-D(0.6 mg L-1)+ 6-BA(0.2 mg L-1).The effect of multiple factors on callus proliferation was better than that of single factors.(4)For the direct induction of adventitious buds of the leaves from tissue cultured seedlings of cultivar 'U-1',the most suitable medium is MS+IAA(0.15 mg L-1)+ NAA(0.05 mg L-1)+ 6-BA(1.0 mg L-1).The induced callus of 'Nongda No.7' did not differentiate into adventitious buds.(5)For the bud induction of the root-like stem of cultivar 'Nongda No.7',the most suitable medium is MS+NAA(0.2 mg L-1)+ 6-BA(0.5 mg L-1).(6)The optimal medium is MS+NAA(0.2 mg L-1)+ 6-BA(0.5 mg L-1)for the rooting of adventitious buds of 'U-1' and the rooting rate is 93.33%.