The Transcriptome Analysis Of Tomato Response To TMV Infection Under High Concentration CO₂

CO2 is an indispensable substrate for plant photosynthesis.At present,the increasing concentration of CO2 in the atmosphere will inevitably affect the growth of plants,but there is little research on the occurrence of plant diseases.In order to determine the effect of high concentration of CO2 on the occurrence of disease and the interaction between plant and pathogen,tomato was used as experimental material to investigate the occurrence of disease in general environment and high concentration CO2 environment.On this basis,the transcriptome analysis of serious diseases and the screening of their effective plant pesticides were carried out.The results were as follows1、the increase of CO2 concentration not only enhanced the plant growth,but also improved the incidence and disease index of F.fulva,B.cinerea、A.solani and tomato mosaic virus,the incidence ofP.macrospora did not change obviously,and tomato mosaic virus was more serious than other diseases in the same growing environment2、A total of 12 samples were sequenced,including C1、C2、C3、T1、T2、T3、CC1、CC2、CC3、TC1、TC2、TC3,and 84.92Gb Clean data were obtained.The percentage of Q30 was above 91.37%and the clean Data of each sample is up to 6.73 Gb.The Clean Reads of each sample was compared with the designated reference genome.The comparison efficiency ranged from 85.79%to 94.51%.Based on the results of comparison,the variable splicing prediction analysis,optimization analysis of gene structure and the discovery of new genes were carried out,thus discovered 1027 new genes.Among them,864 were annotated and 5073 genes were optimized3、Four differentially expressed genes were analyzed by functional annotation and enrichment analysis,found differentially expressed genes involved in plant hormone signal transduction pathways were down regulated,and involved in glutathione metabolism and carbohydrate metabolism and disease resistance related gene expression was up-regulated,so that high concentrations of CO2 and TMV stress stimulated tomato the expression of resistance gene.These transcriptome analysis provide a basis for the study of tomato genome.