Preliminary Study On Mutation Screening Of PS1 And PS2 Genes In Rhesus Monkeys

Purpose: With the improvement of people's living standards,the threat of aging diseases to human health has become more and more serious.In veterinary clinics,pets and laboratory animals are also increasingly threatened by geriatric diseases.PS1 and PS2 are one of the causes of Alzheimer's disease that is currently known to cause age-related degenerative diseases.Based on the research of human Alzheimer's disease-related genes,this study plans to use the traditional sequencing technology to sequence the PS1 and PS2 genes of 271 healthy rhesus monkeys randomly selected,in order to compare the rhesus monkey PS1 and the contrast.The difference between the PS2 gene mutation site and the human pathogenic gene mutation site provides guidance for the quality of experimental animals,the loss of economic animals,the protection of endangered animals,and the clinical aspects of pets.Methods: A total of 271 healthy rhesus monkeys were randomly selected from the study.The peripheral blood cells were extracted and DNA was extracted.The extracted DNA was deeply sequenced,and the detected gene sequence was compared with the gene sequence of the rhesus monkey in the gene bank.Find the corresponding PS1,PS2 mutant gene sequences based on the mutation sites.Results: Each sample was amplified by 13 pairs of primers by sequencing 271 samples.The following mutations occurred in the 271 monkeys that were screened by comparison with the rhesus monkey gene sequence: c.115-117 del AAC,c.C130 T,c.T149 C,c.C164 T,c.G187 C,c in PS1.C255 T,c.C264 T,c.T314 C,c.C854 A mutation;c.A149 G,c.G208 A,c.C217 T,c.G442 A mutation in PS2.Conclusion: Using deep sequencing technology to screen out 9 mutation sites in the PS1 gene of rhesus monkeys and 4 mutation sites in PS2 gene;mistranslation mutations causing amino acid changes may be the main cause of Alzheimer's disease.Factors;lay the foundation for establishing animal models of disease and ensuring the quality of experimental animals.