Germplasm Identification And Transcriptome Analysis Of Saline-Alkali Tolerant Wild Soybean In Eastern Hebei

Soil salinization is a major global problem,and its area is increasing year by year.It seriously affects the development of agriculture.Soil salinization in eastern Hebei Province is serious.In order to further reveal the salt-alkali tolerance mechanism of wild soybean and identify the tolerant to salt-alkali of wild soybean,we determine the content of malondialdehyde,proline,soluble sugar,cell differential permeability and transcriptome level of wild soybean plants with salt-alkali stress,and the transcriptome of two wild soybean materials was analysed by RNA-seq.The results are as follows:1.Identification of 400 wild soybean materials showed that there were 2 materials(Yong2,2010 series 12)with salt tolerance grade 5,accounting for 2%;22 materials with salt tolerance grade 4,accounting for 6%;80 materials with salt tolerance grade 3,accounting for 23%,77 materials with salt tolerance grade 2,accounting for 22%,161 materials with salt tolerance grade 1,accounting for 47%.2.With Saline-alkali stress,the malondialdehyde content of 2010-12 material was the highest,followed by 2010-53 and 2010-74,2012-34 and 2012-49 were least;the proline content of 2010-12 was the highest,followed by 2010-53 and 2010-74,2012-49 was the least;the electrolyte exosmosis rate of 2010-53 was lowest,followed by 2010-12,2012-49 was the highest;the soluble sugar content of 2010-12 was the highest,2012-49 was the lowest.Based on the above results,it showed that 2010-12 was the most resistance material and 2012-49 was the most susceptible material.3.Transcriptome analysis of resistance material 2010-12 and susceptible material 2012-49 under saline-alkali stress showed that the total number of new transcripts was 32331;there were 25536 transcripts with protein coding potential and 7295 transcripts without protein coding potential;there were 23256 transcripts with protein coding potential belonging to known genes and 1780 transcripts with protein coding potential belonging to new genes.There were 8909 differentially expressed genes detected at 0 h,including 5514 up-regulated genes and 3395 down-regulated genes;there were 6512 differentially expressed genes detected at 3 h,including 2335 up-regulated genes and 4177 down-regulated genes;there were 9443 differentially expressed genes detected at 10 h with 4307 up-regulated genes and 5136 down-regulated genes.There are 1770 differentially expressed genes in all three time points.Based on the GO analysis,the most representative biological processes were cellular processes,metabolic processes,biological regulation,stimulation response,cytoplasmic localization and signal molecules.The most representative cell components were membrane,components and cells.The most representative molecular functions were binding,catalytic,transporting,transcriptional regulation and signal transduction.