| Our previous research found that Tachyplesin I(TP I)could improve the growth performance of SPF chickens,promote the production of inflammation and immunosuppressive factors,enhance the body`s immune function,and increase the abundance of short-chain fatty acid-related bacteria,Butyricicoccus and Blautia.Pullorosis disease is a bacterial infection caused by Salmonella pullorum.The chicks often develop acute sepsis after infection.The intestinal structure of chickens infected with Salmonella pullorum is seriously damaged,the microbial micro-ecological structure in the intestinal tract is imbalanced,and the ecological balance of intestinal flora is upset.Regulation of Tachyplesin I on the intestinal flora of Salmonella infection is of great significance for the prevention and purification of Salmonella infection through the intestinal flora.This study investigated the effects of Salmonella pullorum on chicks after intestinal infection,the regulation of Tachyplesin I on the intestinal flora of infected chicks,and the regulation of intestinal microcological disorders by chicken-derived Lactobacillus.The 16 S rDNA of cecal bacteria in infected chicks was sequenced by Illumina Miseq platform which provided data support for enriching the theory of artificial regulation of intestinal flora.The infection of chicks with Salmonella pullorum significantly reduced the growth performance of chicks(P<0.05).Intestinal tissues and structures such as intestinal mucosa and intestinal villi were severely damaged.In addition,liver and lung showed different degrees of lesions.Analysis of the intestinal flora of infected chicks,Salmonella pullorum infection significantly reduced the abundance of bacteria in the intestine,reduced the Firmicutes(P <0.05)and increased Proteobacteria abundance(P<0.05),at the genus level,reduced the abundance of Lactobacillus(P<0.05)and Faecalibacterium(P<0.05)and increased Enterobacteriaceae(P<0.05)abundance level with Oscillospira(P < 0.05).The addition of TachyplesinI to the drinking water of chicken infected with Salmonella was found to increase the growth performance of infected chicks(P>0.05),and it could slow down the damage to the liver and intestines.Tachyplesin I canincrease the species diversity at the level of infected chicks,and can inhibit Lachnospiraceae,Ruminococcus,Coprobacillus,Enterobacteriaceae after Salmonella infection.The trend of bacteria such as Enterobacteriaceae and Clostridiales maintains the stability of the intestinal environment.By adding the chicken-derived Lactobacillus preparation to the infected chicks,it is found that the Lactobacillus-TachyplesinI preparation can improve the microbial diversity in the intestinal tract more than the Lactobacillus alone.Chicken-derived Lactobacillus can restore the abundance of the differential genus Lachnospiraceae and Clostridiales formed by Salmonella infection,but increase the abundance of the abundance of Erysipelotrichaceae,Christensenellaceae,and Peptostreptococaceae.The structure of the intestinal flora was stabilized before 4 weeks of age.In summary,this study demonstrates that Tachyplesin I can promote the growth of chickens infected with Salmonella typhimurium,inflammation and damage in the intestines and liver,and slow the increase and decrease of bacteria such as Lachnospiraceae,Ruminococcus,Coprobacillus,Enterobacteriaceae,and Clostridiales.By supplementing chicken-derived Lactobacillus and Tachyplesin I preparations,the abundance of bacteria in the intestine can be increased,and the abundance level of Lachnospiraceae and Clostridiales of the differential genus can be restored.However,complete restoration of the original structure of the intestinal micro-ecology requires further verification.The data of this study enriched the Salmonella infection and the data theory of the changes of intestinal microflora in infected chicks by the Tachyplesin I,providing a theoretical basis for regulating the intestinal flora stability by Tachyplesin I. |
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