Study On The Relationship Between The Gut Microbiota And The Chicken Infected By Salmonella Pullorum

Pullorum infection in chicks is one of the most common diarrhea related diseases which is harmful to poultry industry.The fundamental way to eliminate pullorum disease is purifying chickesn flock,however,this method has low accuracy,great economic losses and can not effectively control the occurrence of pullorum disease in chickens.Some studies have reported other methods of prevention and treatment of pullorum disease,such as the addition of probiotics,the use of antimicrobial peptides,etc.,but little effect has been achieved in practical application due to the weakness of basic research.With the emergence and development of the nextgeneration sequencing,animal intestinal microbiota have been extensively studied,especially in human gastrointestinal diseases(such as irritable bowel syndrome,inflammatory enteritis disease,etc.).Fecal microbiota transplantation,a new method of treatment,has also shown great promise in the treatment of human intestinal diseases.However,there are few studies on the relationship between pullorum disease and gut microbiota.In this study,the rapid whole blood agglutination test was used to identify 52-week-old Xin Pudong hens.135 positive hens and 140 negative hens were selected to collect the feces,and positive hens were mated with positive hens to obtain positive parents' offspring;negative hens were mated with negative hens,and negative parents' offspring were obtained.Collect the feces of the chicks.A total of 355 samples were obtained.We sequenced the V3-V4 region of 16 S r DNA from the samples.Results are as follow:(1)The intestinal microbiota composition of chickens with different infective characteristics of pullorum was different.The abundance of 39 bacteria were significantly different between chickens negative for pullorum and chickens positive for pullorum(p<0.05),of which 33 genera were extremely significant(p<0.01).(2)Parents with different infective characteristics of pullorum leaded to difference in the intestinal microbial structure of offspring.The potential harmful bacteria abundance of Vibrionimonas,Novosphingobium,Aeribacillus and Corynebacterium in offspring from positive parents were significantly higher than that in offspring from negative parents(p<0.05).(3)The structure and function of intestinal microbial between diarrhea chickens and non-diarrhea chicken were different.3 same potential harmful bacteria in diarrhea chickens and nondiarrhea chickens shows the same results(p<0.05)as positive parents' offspring and negative parents' offspring.The abundance of Hypertrophic cardiomyopathy,Influenza A,Viral myocarditis,Colorectal cancer and Amoebiasis in diarrhea chickens were higher than that in nondiarrhea chickens(p<0.05)after metabolic pathways analysis.In this study,the composition and distribution of microbiota associated with pullorum disease in chickens and functional metabolic pathway were obtained from the intestinal microbiome level,which provided basic data and references for the prevention and treatment of pullorum disease,pathogenic mechanism and host-intestinal microbial interaction mechanism.