The Identification And Analysis Of Grape Housekeeping Gene

Grape(Vitis vinifera L.)belongs to the grapevine grape woody vines,as one of the world's oldest tree species,in the Tertiary strata have been found grape plant fossils.Grape is one of the world famous fruits,and are cultivated all over the world.In 2007,scientists had successfully drawn out the Eurasian grape genome sequence diagram,which was the first time scientists cut the fruit genome code,and the grapes are the fourth sequenced flowering plants.which has greatly promoted the study of grape-related genomes.In order to understand the function of housekeeping genes,it is necessary to screen the butler gene and classify it.Therefore,this study used grape as a test material to screen the housekeeping genes from the perspective of bioinformatics,and to genome analysis of the Actin,GAPDH,TUA and TUB gene families,which was not only for the further understanding of the vines.The function of the gene family provides important sequence information,and through its genetic family gene structure,sequence characteristics and evolution analysis between different species,from the perspective of bioinformatics predicted its potential function for the experimental verification of its genetic function important reference information.1 In order to further understand the gene structure characteristics of stewards in grapes,800 housekeeping genes were found in grapes by referring to previous studies on housekeeping gene recognition methods and using chip data and high throughput sequencing data.Genetic analysis showed that the grape housekeeping gene had a longer gene length,longer coding region,more exons and shorter average exon length than other types of genes,and had a more loose genetic structure.Functional analysis shows that most of the grape housekeeping genes are enzymes that maintain the basic biological activity of the organism.We also identified 20 housekeeping genes for laboratory experiments.2 In this study,the sequence of Actin gene was identified from the whole genome of grape,the gene of Actin gene was identified from the whole genome of the grape,and the gene structure,amino acid characterization,chromosome localization,gene evolution and tissue expression analysis of the Actin gene family were carried out.The gene structure map was constructed by Gene Structure Display Server,and the basic physicochemical properties of amino acids were analyzed by using ProtFaram tool.Protein subcellular localization was carried out by Plant-mPLoc Sever program.The protein subcellular localization was carried out by using the bioinformatics method.SOPMA was used to analyze the protein secondary structure.The three-dimensional structure of the protein was drawn by Phyre2 on-line.Expression pattern at different organizations was analyled based on microarray database of the grape.The result shows that a total of 16 members of the Actin gene family were identified and analyzed according to phylogenetic analysis.Class II and Class III subfamily members have similar genetic structure and amino acid physical and chemical properties.The gene loci showed that the 11 chromosomes of the Actin gene family were distributed in 19 chromosomes,and the distribution of the gene family was extensive.Analysis of different tissue expression profiles showed that Actin gene family members had different tissue expression patterns.In this study,a total of 16 Actin gene family members were identified in grape,which could be divided into three categories,distributed on eleven chromosomes,and the Actin gene expression patterns are specific,and that of information has laid a foundation for the functional analysis of Actin gene family.3 The GAPDH gene sequence was identified from the whole genome of the grape,and the gene structure,amino acid characterization,gene evolution and tissue expression analysis of the GAPDH gene family were analyzed.The gene structure map was constructed by Gene Structure Display Server,and the basic physicochemical properties of amino acids were analyzed by using ProtFaram tool.Protein subcellular localization was carried out by Plant-mPLoc Sever program.The protein subcellular localization was carried out by using the bioinformatics method.SOPMA was used to analyze the protein secondary structure.The three-dimensional structure of the protein was drawn by Phyre2 on-line.Expression pattern at different organizations was analyled based on microarray database of the grape.The result shows that a total of tenmembers of the GAPDH gene family were identified and analyzed according to phylogenetic analysis.The gene loci showed that the 8 chromosomes of the GAPDH gene family were distributed in 19 chromosomes.Cluster analysis showed that the GSVIVG01001842001 and the Arabidopsis AT3G04120.1 and AT1G13440.1 were clustered together,indicating that the genetic relationship was close.Analysis of different tissue expression profiles showed that GAPDH gene family members had different tissue expression patterns.4 In order to recognize the Tubulin gene from the whole genome of the grape,the gene of Tubulin gene was identified from the whole genome of the grape,and the gene structure,amino acid characterization,chromosome localization,gene evolution and tissue expression analysis of the Tubulin gene family were carried out.The gene structure map was constructed by Gene Structure Display Server,and the basic physicochemical properties of amino acids were analyzed by using ProtFaram tool.Protein subcellular localization was carried out by Plant-mPLoc Sever program.The protein subcellular localization was carried out by using the bioinformatics method.SOPMA was used to analyze the protein secondary structure.The three-dimensional structure of the protein was drawn by Phyre2 on-line.Expression pattern at different organizations was analyled based on microarray database of the grape.The result shows that a total of 7 members of the TUA and TUB gene family were identified and analyzed according to phylogenetic analysis.The gene loci showed that the 6 or 7 chromosomes the TUA and TUB gene family were distributed in 19 chromosomes,respectively.Cluster analysis showed that one member of the TUA gene family(GSVIVG01033415001)together with AT5G19770.1(TUA3)and AT5G19780.1(TUA5);GSVIVG01021707001 and GSVIVG01008426001 of the TUB gene family together with AT5G44340.1(TUB4)and AT4G20890.1(TUB9)indicating that the genetic relationship was close.Analysis of different tissue expression profiles showed that GAPDH gene family members had different tissue expression patterns.