Genome-Wide Association Analysis Of Resistance To The Seedling Blast And Panicle Blast In One Rice Natural Population

Rice is one of the main food crops in the world,and the rice blast caused by the fungal Magnaporthe oryzea is one of the major diseases.This disease happens throughout the entire life cycle of rice,and seedling blast and panicle blast are two important types of the disease.It has been proved by the long-term practice that the most economical and effective way to control this disease is identifying new highly and broad spectrum resistant genes and applying them to breed new resistant variety.However,the rice blast fungi are easy to variation,and the varieties with one single resistance gene will be failed to apply after three to five years.Therefore,it is important to continuously identify new genes and breed high resistance cultivar for the disease.Based on huge phenotype data and genetic markers data,the GWAS(Genome-Wide Association Study)method can calculate the SNP sites which are associated with the rice blast resistance.The candidate rice blast resistance genes can be predicted by analyzing the SNP sites and the surrounding sequences.Compared with other traditional methods,this is a time-saving efficient method to identify new rice blast resistance genes.In this study,the 201 seedling blast and the 120 panicle blast sequenced natural rice germplasm were used to identify seedling blast resistance and panicle blast resistance genes by GWAS methods,respectively.The main results are as follows:(1)Seedling blast resistance phenotypes of the population were detected by inoculating two blast isolates Hoku 1 and 40E3.In total,44 associated SNP sites were detected after inoculated with Hoku 1,including 21 sites detected in May and 21 sites detected in October,and 14 sites can detected in both experiments,58 associated SNP sites were detected after inoculated with 40E3,including 48 sites detected at May and 30 sites detected at October,and 20 sites can detected in both experiments.After predicting the candicated gene in theassociated SNP sites,we found of the 14 associated SNP sites with Hoku 1 resistance were previously reported sites,and and the other 6 of the 20 sites were first detected in this study.(2)Panicle blast resistance phenotypes of the population were detected by inoculating two blast isolates Hoku 1.In total,32 associated SNP sites were detected after predicting the candicated gene in the associated SNP sites,the 11 sites were previously reported sites and the other 21 sites were first detected in this studyPanicle blast resistance phenotypes of the population were detected by inoculating two blast isolates Hoku 1.In total,32 associated SNP sites were detected after predicting the candicated gene in the associated SNP sites,the 11 sites were previously reported sites and the other 21 sites were first detected in this study...