| The objective of this study was to investigate the effects of different dietary high fiber levels on growth performance,apparent digestibility of nutrients,species composition and environmental changes in the gastrointestinal microflora of local black pigs,and postintestinal volatile fatty acid content.Through 16 SrDNA high-throughput sequencing technology,the effects of three high-fiber diets on the gastrointestinal microenvironment of local black pigs were analyzed,and the tolerance of local black pigs to high-fiber diets was explored;the establishment of a standard system for black pig rearing was investigated.Provide reliable data.The trial was designed as a single factor,and 36 black pigs were selected according to the principle of consistent health status,genetic background and similar parity.Six black pigs were tested for the apparent digestibility of nutrients using Latin square design,and the remaining 30 were divided into 3 groups(A,B,C,three groups),each group of 10 studies on the growth performance and gastrointestinal microflora changes.The diet was based on a corn-soybean meal type,and three different fiber-level diets were prepared: Crude fiber levels were 9.7%(No.1),14.6%(No.2),and 15.3%(No.3).Research shows1.The crude fiber level of diets had no significant effect on the average daily feed intake of black pigs(P>0.05),but the average daily weight gain of group A and B had significant differences(P<0.05),but A and C were not significant.(P>0.05),B and C were not significant(P>0.05).The feed-to-weight ratio of the experimental group B and C was higher than that of the A group.2.For the apparent digestibility of nutrients,the differences in digestibility of crude fats in the three diets were extremely significant(P<0.01);the digestibility of crude protein and total energy in No.1 material was significantly different from No.2 and No.3 materials.(P<0.05);but there was no significant difference between No.2 material and No.3 material(P>0.05).The apparent digestibility of crude ash,calcium,and phosphorus was significantly different from that of No.1 and No.3 respectively(P<0.05),but there was no significant difference between No.1 and No.3 in this nutrient(P>0.05).3.Among the digestibility of 17 kinds of amino acids,the arginine digestibility of material No.1 was significantly higher than that of No.2 material and No.3 material,but there was no significant difference between No.2 material and No.3 material(P>0.05);There was no significant difference in digestibility of proline,glycine,leucine,tyrosine,phenylalanine,histidine and cystine between the three high-fiber diets(P>0.05);aspartate,The digestibility of threonine,serine,alanine,valine,isoleucine,lysine and methionine showed a significant difference in the digestibility of No.1 and No.2 materials(P<0.05).There was no significant difference in the same amino acids between No.1 material and No.2 material(P > 0.05).4.In the 16 SrDNA high-throughput sequencing experiment,the gastrointestinal microflora of local black pigs was mainly composed of six phyla of Firmicutes,Bacteroidetes,Proteobacteria,Tenericutes,Actinobacteria,and Cyanobacteria.The increase in the level of high-fiber diets led to a significant reduction in the number of colonic bacteria in the colon and increased Bacteroideae.The jejunum and ileum thick-walled bacteria increased significantly.The stomach,cecum,and duodenum;the thickness of the thick-walled bacillus significantly decreases with increasing fiber levels.Intestinal PCo A in the GI tract of the gastrointestinal tract was the most recent and had the strongest similarity;the high-fibre diet had the least effect on the colon and the colonic environment was more stable than other sites.In addition,the Shannon curve values of G1-J,G2-J,and G3-J were the highest,indicating that the species composition of the colon in the gastrointestinal tract is the most complex,and with the increase of dietary crude fiber levels,the species richness of the colon tends to decrease.5.The cecal and colonic E.coli and Lactobacillus results showed that the cecal test group C E.coli and B and A groups were significantly different(P <0.05),A and B groups were not significantly different(P> 0.05).There was no significant difference in the control group A between the Lactobacillus enterococccus,colon E.coli,and the lactic acid bacteria of the colon and the experimental group B and C(P>0.05).The content of valeric acid in group C of cecum was significantly different from that of group A and B(P<0.05).There was no significant difference between group A and group B(P>0.05).There was no significant difference among the other three groups of volatile fatty acids in the other cecum(P>0.05),and there was no significant difference between the volatile fatty acid test group and the control group in the colon(P>0.05). |
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