Heterosis Analysis And Prediction Of Spring Cabbage F1

Cabbage belongs to colewort of cruciferae,a variety of Brassica oleracea L.The use of heterosis can improve the quality of cabbage,enhance the resistance of cabbage and increase the yield of cabbage.In this study,we explored the feasibility of SNP-CAPS molecular marker genetic distance to predict the heterosis of spring cabbage through the selecton and selection of spring cabbage parents,and provided a theoretical basis for the early prediction of hybridization of spring cabbage.In spring cabbage inbred lines were used as parents,and 10 hybrid combinations were combined according to the NCII incomplete double-hybridization group.7 spring cabbage inbred lines were used as parents,and 10 hybrid combinations were combined according to the NCII incomplete double-hybridization group.The variation analysis,correlation analysis,principal component analysis and cluster analysis of 11 agronomic characters of 7 spring cabbage breed materials,and compared the relationship between genetic distance and heterosis of SNP-CAPS molecular marker analysis.The specific results are as follows:(1)There are large variations in the main agronomic traits of 7 spring cabbage breeding materials.Among them,the coefficient of variation of the core length is the largest,and the outer leaf weight is the second.However,the variation coefficient of the transverse diameter of the Head diameter and the core wide is small,and the selection range is not large;the coefficient of variation of other traits is large and can be selected.(2)Through principal component analysis,converting the main agronomic characters of spring cabbage into 6 principal components,the cumulative contribution rate of the first three factors reached 85.105%.It shows that the first three principal components extracted are relatively high.The comprehensive information it conveys reflects most of the genetic information of the main characters.It provides a theoretical basis for the selection of spring cabbage varieties.(3)Cluster analysis,based on the different performances of 11 agronomic characters among 7 spring cabbage parent materials,The test materials are grouped into three groups at the Euclidean distance of 10,Group I consists of O-D6-1,98-1030,16-1003 and RM.Group II is only8-1005,and Group III contains QM and Q4.For certain characters,Found to be based on the distance of the genetic relationship or the size of the genetic distance,on certain of up prediction heterosis.(4)In heterosis mid-parent in the head weight.Through heterosis analysis,it was found that the RM×98-1030 head weight had the highest mid-parent,high-parent and heterosis index.RM×98-1030,QM×O-D6-1 and QM×8-1005 have certain value in use,genetic improvement of these spring cabbage varieties can be utilized.(5)The head weight of RM and Q4 has the best performance.The head weight general combining ability of O-D6-1 performs well,and the other characters have excellent comprehensive performance.It can be used as a excellent parent for further utilization;QM×8-1005 head weight specific combining ability is best,the head weight specific combining ability of RM×16-1003,RM×98-1030 and RM×Q4 is better,and the comprehensive performance of other characters conforms to the excellent combination standard,and can be used for improvement.(6)Cluster analysis of SNP-CAPS molecular markers divided 7 spring cabbage parent materials into 3 groups,the group?16-1003,RM and 8-1005,the group?QM and Q4,the group?O-D6-1 and 98-1030.SNP-CAPS molecular marker analysis The genetic distance of the seven experimental parental materials varied from 0.41 to 0.82,the results showed the probability of strong superiority crosses of advantages is greater.(7)According to the analysis of combining ability variance and SNP-CAPS molecular marker clustering,it was found that the hybrid combinations RM×98-1030 and RM×Q4 of RM,98-1030 and Q4 were the most likely to be strong superiority crosses,the results showed that molecular markers can predict heterosis.(8)According to the analysis of combining ability variance and SNP-CAPS molecular marker clustering,it was found that the hybrid combinations RM×98-1030 and RM×Q4 of RM,98-1030 and Q4 were the most likely to be strong superiority crosses,the results showed that molecular markers can predict heterosis.