Effects Of Cinnamic Acid Treatment On Physiological And Biochemical Basis Of Pugionium Gaertn Seedling

Pugionium Gaertn.is a endemic genus of sandy land in the Mongolian Plateau in central Asia,belong to P.coenutum and Rdolabratum,it is a kind of sandy vegetable with high nutritional value,but the self-poisoning of mustard plants not only brings great obstacles to its introduction and domestication and cultivation,it also restricts the distribution and dissemination of wild plant resources,which is not conducive to their original protection and utilization.Several autotoxic substances have been isolated and identified from the Pugionium Gaertn plants in our laboratory,for this reason,this experiment used P.coenutum and P.dolabratum as test materials,different concentrations of cinnamic acid were used to treat the seedlings of P.coenutum and P.dolabratum.Effects of different concentration of cinnamic acid on physiological,biochemical and soil environment of Pugionium Gaertn seedlings.This study provided a theoretical basis for the conservation of resources,introduction and domestication of Pugionium Gaertn plants and the cultivation of new varieties.The main research results are as follows:1.With the increase of cinnamic acid concentration,root length,leaf length,fresh weight of root and leaf,dry weight of root and leaf and root activity of the P.coenutum and P.dolabratum decreased gradually,when the concentration of cinnamic acid was more than 0.01 mmol/L,it began to decrease significantly,the concentration of cinnamic acid at 10 mmol/L was the lowest,the results showed that cinnamic acid concentration greater than 0.01 mmol/L could inhibit the growth of seedlings and root absorptive capacity of Pugionium Gaertn Seedling.2.With the increase of cinnamic acid concentration,the activities of SOD,POD and CAT in the roots and leaves of P.coenutum and P.dolabratum showed a trend of first increasing and then decreasing,when the concentration of cinnamic acid was lower than 0.1 mmol/L,the enzyme activities of P.coenutum and P.dolabratum increased,while those of Mustard axe were higher than 0.1 mmol/L decreased;The contents of proline,MDA and conductivity in the root and leaf of P.coenutum and P.dolabratum increased with the increase of cinnamic acid concentration,which indicated that cinnamic acid could increase the peroxidation degree of plasma membrane,reduce the osmotic regulation ability of Pro and destroy the permeability of cell membrane.3.With the increase of cinnamic acid concentration,the net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),light energy conversion rate(Fv/Fm)and electron transfer efficiency(PS Ⅱ)of leaves of P.coenutum and P.dolabratum were increased,photochemical quenching coefficient(qp)and non-photochemical quenching coefficient(NPQ)gradually decreased;Intercellular CO2 concentration first decreased and then increased;Chlorophyll a,chlorophyll b and chlorophyll a+b in leaves of Rcoenutum and P.dolabratum showed a decreasing trend.The effect of cinnamic acid on Photosynthetic Characteristics of P.dolabratum was lower than that of P.coenutum.4.With the increase of cinnamic acid concentration,the number of bacteria,fungi and actinomycetes increased first and then decreased,while the activities of urease,invertase and alkaline phosphatase increased first and then decreased;Soil total nitrogen,available nitrogen of P.dolabratum and available potassium of P.coenutum increased gradually;Available phosphorus,potassium of P.dolabratum and total nitrogen in P.coenutum soil decreased gradually;Available nitrogen in P.coenutum soil increased first and then decreased;Available phosphorus in P.coenutum soil decreased first and then increased;With the increase of cinnamic acid concentration,the total nitrogen and potassium in the root and leaf of P.dolabratum decreased first and then increased;Total potassium increased in P.dolabratum;Total nitrogen and potassium in root and leaf of P.coenutum increased first and then decreased;Total phosphorus in the root and leaf of P.coenutum increased gradually.