Multi-algorithm Synergy Of Orthologous Genes In The Application Of NF-Y Gene Family In Tea Plants

Bioinformatics is a research field that makes full use of computers,biology,informatics,mathematics,statistics and other tools to effectively analyze large amounts of biological data and ultimately obtain valuable and meaningful biological information.The study of gene families of species based on orthologous genes and multi-algorithms has always been the focus and hotspot of bioinformatics,and the prediction of gene family biological functions based on transcriptome data is also an extremely effective means of analysis.The NF-Y genes is a transcription factor in plants and plays an important role in the growth process and stress resistance of plants.However,the existence of NF-Y genes in tea plants and its biological functions have not been discovered.Based on the background of this research,our study based on the whole genome of tea tree and NF-Y orthologous gene using a combination of various algorithms to carry out multi-level research and analysis on the NF-Y genes family of tea tree,and effectively predicted its biological function.In this study,the genomic,whole-tissue and multi-environmental transcriptomes of tea trees were combined with various algorithms to mine and identify the NF-Y gene family.At the same time,the species evolution and differentiation time of the tea tree NF-Y gene family were effectively analyzed,and the biological function of the tea tree NF-Y gene was reliably predicted based on the phylogenetic tree constructed by the orthologous gene.Finally,based on the transcriptome data in various states of tea tree,the transcriptome data was analyzed by using multiple algorithms.According to the analysis results,the biological function of tea tree NF-Y genes family was further predicted.The main results are as follows:1.The NF-Y genes family was obtained by combining various algorithms in tea tree genome data,including 9 NF-YA genes,16 NF-YB genes and 10 NF-YC genes.Simultaneously,the study also showed that the sequence of the tea tree NF-Y gene family is highly conserved.2.The physicochemical properties and structure of the tea tree NF-Y genes family were analyzed by multiple algorithms.The results showed that the tea tree NF-Y gene proteins is a hydrophilic fat-soluble stable protein,and the predicted tertiary structure is highly reliable.3.Based on the orthologous gene analysis of the differentiation time and phylogenetic tree of NF-Y gene family of tea tree,it was found that the replication of NF-Y gene in tea tree probably occurred between 32.18 million and 3.9 million years ago.And the differentiation of tea and kiwifruit was found between 49 and 13 million years ago.Through the phylogenetic tree,we predicted that the tea tree NF-YA2 and NF-YA8 genes are involved in the drought-resistant process of tea trees and that NF-YB12,NF-YB14 and NF-YC7 are involved in the process of promoting tea planting.4.Based on tea tree transcriptome data,the biological function of tea tree NF-Y gene family was predicted.We predicted that four genes such as NF-YA6 in tea tree NF-Y gene family play an effective role in the growth of tea tree against cold stress.Seven genes,such as NF-YA5 and NF-YA7,participated in the drought-tolerant regulation mechanism of tea trees in drought stress.