Effect Of Genetic Background On The Function Of Rice DEP1 And The Polymerization Effect Of DEP1 And IPA1

Plant type improvement has always been an important way to increase yield potential in rice.With the development of molecular biology,genes related to many plant type traits in rice have been cloned one after another.Among these genes,the erect panicle gene DEP1 increased the number of branches and the number of grains per panicle,and increased nitrogen use efficiency,which was widely used in northern japonica rice breeding.The main characteristics of the ideal plant type gene IPA1 are less tillering,no ineffective tillering,thick stems,and large spikes.The influence of genetic background on the function of DEP1 and its interaction with IPA1 is a problem worthy of further study in rice plant type improvement.The research results are as follows:1.The 35 S overexpression vector was constructed by the erect panicle allele dep1,and transformed into rice Akita Komachi and Liaogeng No.5 by Agrobacterium-mediated transformation,and the dep1 transgenic line in the background of Akita Komachi and the dep1 in the background of Liaogeng No.5 were bred.The transgenic line was tested for its plant type,leaf area,physiological index,material production and yield.Due to the overexpression of dep1 gene,the ox-dep1 in the Akita Omachi was significantly lower than that in the wild type after 20 days in the heading stage;the length of the flag leaf,the length of the 2 leaves,and the length of the 3 leaves were significantly decreased.The width decreased,the width of the inverted 2 leaves increased significantly,and the width of the inverted 3 leaves decreased significantly;the curvature of the neck decreased significantly;the angle of the flag leaf,the angle of the inverted 2 leaves,and the angle of the inverted 3leaves decreased;the value of SPAD increased;the index of nitrogen balance increased;The output is increased.However,under the background of Liaogeng No.5,the plant height of transgenic rice showed an upward trend,and only the plant height of LD4 plants decreased;SPAD and nitrogen balance index were higher than wild type Liaogeng No.5 in the first 25 days of the initial heading stage;the yield increased significantly.In different genetic backgrounds,the expression of dep1 gene function is affected by genetic background,and the phenotypes of different backgrounds are different,even diametrically opposite.For example,the ox-dep1 transgenic line showed a downward trend in the background of Akita Komachi,but The plant height of ox-dep1 transgenic lines in the background of Liaogeng No.5 was on the rise;the grain width was decreased in the background of Akita Komachi,and the grain width was increased in the background of Liaogeng No.5.In the background of Akita Omachi,the ox-dep1 transgenic lines showed a significant decrease in the length of the leaves,the length of the 2 leaves,and the length of the 3 leaves.The flag leaf length of the LD1 background dep1 transgenic line LD1 was significantly decreased,and the LD3 and LD4 transgenic lines were 2 leaves long.Significantly decreased,the LD2,LD3,LD4,LD5 transgenic lines decreased significantly.2.The dep1 transgenic line and the ipa1 transgenic line under the background of Fengjin were constructed respectively.The dep1 transgenic line and the ipa1 transgenic line in the background of Fengjin were used as parents,and the dep1 gene and ipa1 gene polymerization system were obtained.The plant height of dep1 transgenic lines was significantly lower than that of Fengjin,and the plant height of ipa1 transgenic line was slightly lower than that of Fengjin.The gene polymerization line was significantly lower than Fengjin before 20 th jointing stage,and the plant height was significantly higher than that of Fengjin and its parents after 20 d.The dep1 transgenic line in Fengjin background and the ipa1 transgenic line in Fengjin background.In the background of Fengjin,the dep1 transgenic line,the ipa1 transgenic line,the parent of the polymer line,the inverted 2 leaves,and the inverted3 leaves grew longer and the leaf width became narrower.The degeneration of the dep1 transgenic lines was significantly reduced,the flag leaf angle was significantly decreased,the angle between the inverted 2 leaves was significantly decreased,and the angle of the inverted3 leaves of the dep1 transgenic line was decreased.The curvature of the neck of the ipa1 transgenic line was significantly increased,the flag leaf angle was significantly decreased,the angle of the inverted 2 leaves of the ipa1 transgenic line was significantly decreased,and the angle of the inverted 3 leaves of the ipa1 transgenic line was decreased.In the gene polymerization system,the curvature of the neck was lowered,the flag leaf angle was increased,the angle of the inverted 2 leaves of the gene polymerization system was significantly decreased,and the angle of the inverted 3 leaves of the gene polymerization system was decreased.Under the background of Fengjin,the leaf area index of the ipa1 transgenic line in the background of Fengjing was significantly lower than that in the background of Fengjin.The leaf area index of the gene polymerization system was slightly lower than that of the wild type Fengjin,but it was significantly higher than that of the dep1 transgenic line in the background of the parents.Ipa1 transgenic line under the background of brocade.The nitrogen balance index of the gene polymerization system was significantly higher than that of the two parental transgenic lines and the wild type Fengjin.The nitrogen balance index of the dep1 transgenic line in the Fengjin background was significantly higher than that of the wild type Fengjin.The ipa1 transgenic line in the Fengjin background was slightly higher than the wild.Type Feng Jin.The biomass of the gene polymerization system increased,but the economic coefficient decreased.The yield per plant was higher than that of the wild type and the parents.The number of panicles per well,the seed setting rate and the1000-grain weight decreased,but the number of grains per panicle increased.The two dominant genes widely used in the breeding of two indica rice were polymerized into the indica rice cultivar,and the obtained gene polymerization line phenotype was a combination of parental phenotypes,which was superior to the parents and wild type,showing less cockroaches,strong stalks,and large The ear and erect panicles have compact plant type and increased yield,which provide theoretical basis and practical basis for high-yield and super-high yield molecular breeding of rice.