Research On The Differential Photosynethic Protein And Gene In The First Generation Of Oryza Sativa

The space environment has the characteristics of low temperature,strong radiation and microgravity,which plays an important role in the study of biological adaptation in space and variation of organisms and mutation breeding.A large number of flight tests have shown that the environment in space will have different effects on the phenotype,growth state,metabolic process and gene protein expression status of organisms.Rice is a gramineous plant.It has become a typical model plant because of its relatively small genome,low introns,high gene density and short growth cycle.In order to explore the effects of spaceflight on plants’ photosynthesis and to explore the molecular mechanism of biological effects,this research carried the seeds of rice Dongnong 423 and Dongnong 416 loading on the recovery satellite Shijian10 and estimated the physiological indicators,expression of protein and gene of rice seedlings in F1 generation.The results were compared and analyzed in the generation of F1 and F0,and the photosynthesis protein and gene variation caused by space flight were studied in a multi-faceted manner.The following results were obtained:The physiological characters of F1 generation of rice Dongnong 416 and Dongnong 423 after space flight was determined and the physiological changes were analyzed.The results showed that the germination ability and agronomic traits of F1 rice seeds did not change significantly after spaceflight compared with the control group,while the photosynthesis metabolite content changed greatly.The photosynthetic pigment content of Dongnong 423 decreased at first and then increased.Dongnong 416 is a downward trend,and the change of photosynthetic metabolite content of F1 generation showed a regression trend compared with F0 generation,and the change of chlorophyll content has a certain genetic tendency.The protein expression of the F1 generation in the three-leaf stage and the tillering stage of two rice cultivars flying through space was detected by iTRAQ technology.The photosynthesis-related differential proteins were analyzed by bioinformatics technology,and the expression of F0 protein expression was analyzed..The differentially expressed proteins in the F1 generation of the two rice varieties were more than the tillering stage in the three-leaf stage,and the number of up-regulated proteins in the three-leaf stage was higher than that in the three-leaf stage.There were 10 photosynthesis-related proteins in the three-leaf stage of Dongnong 416.The expression levels of 6 proteins were up-regulated and 4 were down-regulated.Six photosynthesisrelated proteins were differentially expressed in Dongnong 416,and the expression levels of 4 proteins were up-regulated.Down-regulation;9 photosynthesis-related proteins were differentially expressed in the three-leaf stage of Dongnong 423,6 protein levels were up-regulated,and 3 were down-regulated;3 photosynthesis-related proteins were differentially expressed in Dongnong 423,and all were down-regulated.The differences of photosynthetic proteins between F1 and F0 were analyzed.Only one protein,ferredoxin,showed significant down-regulation in both F1 and F0 generations,and its changes had a genetic trend.Fluorescence quantitative RT-PCR was used to verify the protein coding genes of the two rice cultivars F1 that showed significant differences in space flight,and the expression of the key protein encoding genes of photosynthesis was not changed.The expression of gene in F1 generation after spaceflight at Dongnong 416 was up-regulated,while the expression of Dongnong 423 were down-regulated.At the same time,the trend of protein expression was not consistent with the trend of gene expression.The effect of space flight on the gene was more than the effect in protein.Combined with the changes in protein expression and gene expression,the key sequence of the key enzyme of the photosynthetic key protein dark reaction was cloned,and the gene coding sequence was cloned.No significant differences were found in the analysis of the gene sequence and promoter sequence.