Preparation Of Microbial Compound Agent For Nitrogen-retaining During Livestock And Poultry Waste Composting

Aerobic composting is an important way to realize the utilization of livestock manure resources,but serious nitrogen loss is the biggest problem facing composting.In order to reduce the ammonia emission and reduce the nitrogen loss during the composting process,the traditional microbiological method was used to screen the nitrogen-transforming functional strains close to the environment from the natural environment.By studying the interaction between the various strains,the combination was optimized and one was obtained.The compound microbial agent with better nitrogen retention effect was studied,and the optimal inoculum amount of the composite microbial agent was studied,in order to provide microbial materials and technical support for the regulation of nitrogen loss during aerobic composting and the production of high quality bio-organic fertilizer.The main conclusions are as follows:(1)From the natural environment,4 strains of strains(Am11,Am13,Am15,Am17),3strains of bacteria(Ni8,Ni14,Ni17),and 3 strains of bacteria(Nt1,Nt6,Nt8)were obtained by repeated screening.Five strains of bacteria(Az1,Az9,Az13,Az16,Az17),and thus constitute a nitrogen-transforming flora.(2)The ? group of the present group have better nitrogen-fixing effect and better compost quality.The total ammonia emission of inoculated ? strains during composting was *% to *% lower than that of other groups,and the maturity cycle was shortened by more than * days.At the end of composting,the HA/FA value reached *,the highest degree of humification,and total nitrogen.The content and total nutrient content were *% and *%,respectively,which were *% to *% and *% to *% higher than other groups.It was indicated that the * strain has the best nitrogen retention effect and can shorten the composting cycle,which was a better nitrogen-fixing composite fungicide.(3)Inoculation of ? nitrogen-fixing compound bacteria can effectively improve the microbial community structure and increase microbial diversity.The horizontal microbial abundance showed that the microbial advantage in the No.? heap was obvious during the high temperature period and the end of composting;the OTU,Chao1 index andobserved-species index in the No.? pile and the blank pile at the end of composting compared with the initial stage.The increase were *%,*%,*% and *%,*%,*%,and the Shannon index and Simpson index increased by *%,decreased by *%,decreased by *%,and increased by *%.It indicated that the richness and diversity of microbial communities in the heap inoculated with compound No.* were higher than those in the blank control.(4)The preferred bacterial inoculation amount of the composite microbial agent was*%.Compared with other inoculation doses,* of the inoculum composting process reduced total ammonia emissions by *% to *%;at the end of composting,the HA/FA value reached *,the highest degree of humification,and total nitrogen content and total nutrients The contents were *% and *%,respectively,which were *% to *% and *% to *% higher than other inoculums.It indicated that the inoculation amount of the composite microbial agent was *%,the nitrogen retention effect was the best,and the humification degree and nutrient content of the heap were higher,which was a better inoculum.