Effects Of Spermine On Polyamine Deposition Of Different Tissues,Polyamine Metabolism Of Small Intestine And Immune Function In Geese

Polyamine is a polycationic aliphatic compound necessary for cell growth,and is widely involved in regulating various physiological and pathological processes such as animal reproduction,immunity,intestinal development,digestion,absorption,aging,and cancer.In recent years,studies have shown that spermine is one of the essential factors for cell proliferation and differentiation.It not only participates in the regulation of animal epithelial cell proliferation and apoptosis,promotes intestinal development,but also participates in the regulation of intestinal digestion,absorption and immune function.However,studies on the effects of exogenous spermine on polyamine deposition,intestinal polyamine metabolism and immune function in goose tissues have not been reported.Therefore,this study used different concentrations of spermine to feed adult Sichuan white goose female geese.High performance liquid chromatography and real-time quantitative PCR were used to detect polyamine content,polyamine metabolism and immune related factor gene expression in goose.The regulation of exogenous spermine on polyamine deposition,intestinal polyamine metabolism and immune function in goose tissue,in order to lay a foundation for the study of spermine biological function,and provide a new way for the development of new functional feed.The main results of this study are as follows:(1)Spermine gavage significantly reduced the content of putrescine in the brain and cerebellum of the goose and the content of spermidine in the pectoral muscle tissue(p < 0.05);significantly increased the content of putrescine in the breast muscle tissue of the goose and the cerebellum and gland Spermidine content in the stomach and leg muscle tissues(p < 0.05);significantly increased spermine content in the brain,cerebellum and liver tissues of the female goose(p < 0.05).(2)The gene expression levels of AZIN1,OAZ1,SPMS,SSAT and APAO in the duodenum of 5 mg/kg spermine gavage group were significantly higher than those in the control group(p < 0.05);The expression of jejunal tissue AZIN2,OAZ1 and OAZ2 gene was significantly higher than that of the control group(p < 0.05),and the expression of APAO gene was significantly lower than that of the control group(p < 0.05).The expression levels of AZIN2,SPMS and SSAT in ileum were significantly higher than those in the control group(p < 0.05);the content of spermidine and spermine in the duodenum and jejunum was significantly higher than that in the control group(p < 0.05).The expression levels of AZIN1,OAZ1,ODC,SPDS and SSAT in the duodenal tissues of 10 ng/kg spermine fed group were significantly higher than those in the control group(p < 0.05);The expression of jejunum AZIN2,OAZ2,ODC,APAO and SMO gene was significantly higher than that of the control group(p < 0.05).The expression of SPDS gene was significantly lower than that of the control group(p < 0.05).The expression levels of AZIN2,ODC,SPDS,SPMS and APAO were significantly higher than those of the control group(p < 0.05);the content of putrescine in jejunum and ileum was significantly lower than that in the control group(p < 0.05).(3)The liver index of goose in the 10 mg/kg spermine-gavage group was significantly lower than that in the control group(p < 0.05);5 mg/kg spermine-gavage group was used for goose thymus and bursal TNF-?,IL-1?,IL.The expression of-6 and IFN-? was significantly lower than that of the control group(p < 0.05);the expression of TNF-? and IFN-? and the expression of IFN-? in the liver of 10 mg/kg spermine fed group were significantly lower than those in the spermine-gavage group.The control group(p < 0.05);exogenous spermidine significantly down-regulated the expression of IFN-? in the spleen(p < 0.05);the spermine content in the spermine-gavage group was significantly higher than that in the control group(p < 0.05).In summary,exogenous spermine significantly changes polyamine content in different tissues in female geese,and affects polyamine metabolism of goose small intestine by regulating the expression of key enzyme genes related to polyamine metabolism.In addition,spermine participates in the regulation of goose immune function by mediating the expression of TNF-?,IL-1? and IL-6,IFN-? genes.