The Relationship Between Learning And Memory Of Related Information Materials Of Monochamus Alternatus By Scleroderma Sichuanensisand With CREB Gene Expression

In the face of complex and ever-changing environmental information,parasitic wasps use the chemical information substances in the environment to improve the efficiency of finding hosts by learning behaviors.In the context of biological control,how to use parasitic wasps to improve their research on the parasitic rate of drill collar pests is urgent.This experiment is based on the learning behavior of Scleroderma sichuanensis Xiao.The study was conducted with different learning stages of the female wasp,and the behavioral analysis was used to identify the different stages.The transcriptome sequencing was carried out,and CREB gene screening was carried out based on this.The two CREB genes of S.sichuanensis were successfully cloned by RT-PCR.The CREB1 ? CREB2 gene was analyzed by fluorescent quantitative PCR in different stages.The transcriptome of S.sichuanensis was also analyzed.The expression of the CREB gene in different states and different learning stages was analyzed.The research data support for the learning and memory mechanism of S.sichuanensis.Result:(1)In the study of the behavior of S.sichuanensis,it was found that different kinds of volatiles have different effects on the learning effect of S.sichuanensis,and the effects of different concentrations of volatiles are different.?-pinene,?-pinene,eucalyptus,limonene,and 3-carene were all attractive to the S.sichuanensis with learning experience.?-lauretene had obvious repellent effect on S.sichuanensis,and enhanced the repellent effect of?-lauroene on S.sichuanensis with learning experience.(2)There were 190451 Transcript sequences and 110003 Unigene sequences in the Transcriptome.The Unigene sequence of transcriptome data was compared by database,54349 NR database annotations,40 917 NT database annotations,29,522 KO database annotations,44,034 notes in SwissProt database,48,133 PFAM database annotations,48,815 GO database annotations,KOG database annotations 28521 article.The results of GO database annotation showed that the number of process genes,cell genes and binding genes were 30697,18573,29319,respectively.The KOG database annotation results show that the number of general function prediction is 3995,the number of signal transmission mechanism is 3673,the number of post-translation modification,protein conversion and the partner have 3133.,which together accounted for 37.87% of all unigenes.The unigenes involved in cell processes,environmental information processing,genetic information processing,metabolism,and organic systems were 9078,8447,6032,5198,5048,respectively.According to the difference analysis of gene expression,there were significant differences in the expression of 109 genes including some CREB genes in different states of S.sichuanensis.(3)Two CREB gene sequences of S.sichuanensis were cloned.The CREB1 gene encodes 443 amino acids with a predicted protein molecular weight of 48.06 kDa.a similar rate to the CREB gene of Polistes dominula of 77.27%.the CREB2 gene encodes 101 amino acids with a predicted protein molecular weight of 12.07 kDa.a similar rate to the CREB gene of Solenopsis invicta Buren 74.14%.(4)The expression of CREB1 and CREB2 in female wasps of different learning stages was detected by fluorescence quantitative PCR.The results showed that the expression of CREB1 and CREB2 had the same trend as the learning and memory effect of female wasps.That is to say,with the increase of the learning stages of S.sichuanensis,they all showed the trend of increasing first and then decreasing,It reached its highest level on the 6th day,which indicated that the learning and memory of S.sichuanensis were correlation with CREB gene expression.