| Soybean borer [Leguminivora glycinivorella(Mats)] is one of the most important pests in soybean production.It belongs to diapause insects and has a single feeding habit.It only harms cultivated soybean,wild soybean and Sophora flavescens.Feeding larvae within the pods develops and harms the kernels,severely affecting soybean yield and quality.Breeding and utilization of insect-resistant varieties have long been considered as the most economical and effective way to control pests.The use of resistant varieties to control pests is of great importance for maintaining the ecological environment,improving product quality,and ensuring food safety.It is in line with the green sustainable development strategy of modern agriculture,and people pay more attention to the selection and breeding work of soybean anti-heart borer varieties,and clear varieties.The characteristics of the resistance mechanism of the carnivorous insects is a prerequisite for good resistance to borer breeding.In this article,160 soybean cultivars in Northeast Soybean were screened for resistance factors.Systematic study of the relationship between single factor and its resistance to carnivorous insects such as epidermal features,pod structure,and molting content.The mechanism of the resistance of soybean pods to soybean borers was initially clarified,and the concept of relative resistance of group cultivars was proposed.Using the "relative resistance coefficient method" to evaluate the level of resistance of existing species to the heartworm.It provides scientific basis for the breeding of soybean anti-heartworm species and evaluation of insect resistance.The specific findings are as follows:1.A representative sample of 160 soybean materials was collected and identified in the central region of northeast China.and the characteristics of pod hair density,pod length,pod hair color,and state of life were observed.At the same time,the thickness of each layer was measured under the microscope,and the distribution law of these characters was fitted.Using the significance analysis of each trait,a total of 45 gradient cultivars of each trait were screened as materials for studying the structural resistance mechanism of pods.2.The apparent characteristics of pod showed significant effect on oviposit selection.In the same species of plant,a different section in a significant differences amount pod ovum,significantly lower than the lowest amount of upper bean plant with eggs,and adults prefer to lay their eggs in 3 ~ 5 cm long pods,drum grain of relatively full pods.The phenotypic traits of different pods of different pods had a significant effect on the ovipositivity of the adult worms.The adult species preferred to lay eggs in pods with large density and deep color,and the model for the density adaptability of pod was Y=6.22/(1+EXP(3.17-0.88X)),which was significantly positive.Not pod for pod length of wool,wool more long more easily with eggs,suitable for the spawning of pod length of wool between 2.00-2.10 mm,its selective model for model for single pod egg quantity Y=-23.98+27.82X-6.89X2(F=6.0008,P=0.0624,R=-0.8660)were significantly negative correlation.3.The different anatomic layer thickness of soybean molting had a significant effect on the rate of penetration of newly hatched larvae.Through the “islanding” test,the success rate of newly hatched larvae entering into soybean meal was observed.The epidermis cuticle and subcutaneous thick-walled cell layer in the molted skin had no significant effect on the entry of the newly hatched larvae.The thickness of mesocarp cell layer and inner wall cell layer had a significant effect on the incubation of newly hatched larvae,and had a significant negative correlation with the introgression rate.The R values were-0.9738 and-0.9709,respectively,indicating the thickness of the pericarp and inner cell layer in the soybean pod skin.The main factors affecting the incubation of the newly hatched larvae into the moltings are their adaptive models:Y=142.94EXP(-0.7026X)(F=91.7777,P=0.0002,R=-0.9738)?Y=103.64/(1+EXP(-5.28+20.29X))(F=24.6553,P=0.0137,R=0.9709)?4.The contents of cellulose,hemicellulose,lignin and silicon in soybean molt were negatively correlated with the incubation rate of newly hatched larvae.The correlation coefficient R values were:-0.8359,-0.6932,-0.8623,-0.5508,respectively.indicating that the content of these substances is increased,and the rate of incubation of newly hatched larvae will be reduced accordingly,which will have an impact on the larvae of newly hatched larvae.5.The mechanism of resistance of soybean pods to spawning selection and larvae pupa was firstly clarified,and the relative resistance coefficient method was used to evaluate the resistance of the cultivars.The analysis of the results of the insecticide rate and the resistance coefficient of the 45 species showed that the correlation between the relative resistance coefficient method and the actual field insecticide rate was-0.717,reaching a significant level.It shows that “relative resistance coefficient” can be used as an indicator to evaluate the resistance level of the local population or strain to the carnivora. |
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