Study On The Anti-Diarrhea Mechanism Of Macleaya Cordata Extract In Model Mice

Diarrhea is an intestinal disease caused by a variety of pathogens and factors.Although the use of antibiotics can relieve the occurrence of diarrhea to a certain extent,the excessive use or abuse of antibiotics will lead to increased bacterial resistance in the gastrointestinal tract of animals,drug residues and environmental pollution.There will affect the safety of animal origin food and directly affects human health and environmental safety.Therefore,more and more researchers are focusing on natural herb extracts.The extract of Macleaya cordatahas has anti-inflammatory and anti-bacterial effect,which has a significant effect on relieving diarrhea in animals.However,the mechanism of its intervention has not been reported.In this research,the UPLC-QTOF-MS metabolomics method was used to study the regulation of Macleaya cordata extract on the serum and urine metabolites of diarrhea mode mice,and the mechanism of the intervention of Macleaya cordata extract interfere with diarrhea.The main research contents and results are as follows:1.Observation of the effects of Macleaya cordata extract in diarrhea mode mice which is induced by the leaf of Folium sennaeFifty SPF Kunming mice were selected and divided into Model group,Low dose group of Macleaya cordata extract,Middle dose group of Macleaya cordata extract,High dose group of Macleaya cordata extract,and Positive drug group(berberine hydrochloride group),randomly 10 mice per group.After grouping,each group was given the leaf of Folium sennae.The model group was given saline by gavages,and different dose group of the Macleaya cordata extract was intragastrically given Sangrovit(low dose=105mg /kg.d,medium dose = 315 mg /kg.d,high dose = 1050 mg/kg.d).All groups of mice were checked for general condition and diarrhea index,which was used to evaluate the effect of Macleaya cordata extract in diarrhea mice.The results showed that after successful made the diarrhea models,the mice's anus was accompanied by feces,the fur was messy and the weight was lighter than the blank group,and the spirit is more tired and fatigued.After the drug treatment,no more loose stools,hair gloss returned to normal and the mouse showed excitement.Calculating diarrhea index can be found that the high dose group and berberine hydrochloride group had significant differences with the model group.2.Untargeted metabolomics study on the intervention of Macleaya cordata extract in diarrhea mode mice which is induced by the leaf of Folium sennae based on UPLC-QTOF-MS analysisForty SPF Kunming mice were randomly divided into blank group,model group,Macleaya cordata extract group,and positive drug group(berberine hydrochloride group),ten in each group.Senna models were used for the model group,the Macleaya cordata extract group,and the positive drug group mice.After successful model establish,the blank group and model group were given normal saline.The Macleaya cordata extract group was given Sangrovit(1050mg/kg.d).The berberine hydrochloride group was given berberine hydrochloride by gavage(100mg/kg.d),once a day for three days.Multivariate statistical analysis was used to analyze serum and urine metabolic profiles and metabolic changes in mice.The results showed that 15 differential metabolites were found in serum by differential analysis and 16 differential metabolites were found in urine.By analyzing these metabolites,it can be speculated that the process of diarrhea mainly causes the body's imbalance of amino acid metabolism,TCA cycle,and dopamine degradation,and produces a series of inflammatory substances including leukotrienes and prostaglandins.However,the extracts of Macleaya cordata mainly relieve diarrhea through the catecholamine biosynthetic pathway.In summary,Macleaya cordata extract have obvious improvement on the general behavior of mice with diarrhea.Macleaya cordata extract has a benign regulatory effect on key metabolites of diarrhea in mice.Through the metabolomic study of serum and urine,it was found that the mechanism of diarrhea was improved by the extract of Macleaya cordata which may be through the regulation of dopamine metabolism to relieve diarrhea.The results of research provides a scientific evidence for disease prevention of traditional chinese medicine in the process of livestock and poultry breed,especially forunderstanding of diarrhea prevention mechanism.