Identification And Functional Analysis Of MicroRNA Response Of Black Spot In Panax Notoginseng

Plant microRNAs(miRNAs)are a class of 18-24 nt non-coding RNAs that are involved in the regulation of plant growth and development,reproduction and genomic recombination.At the same time,microRNAs also play a role in plants responding to biotic and abiotic stresses and enhancing tolerance.Black spot is one of the most vulnerable disasters during the growth of Panax notoginseng.It often causing immeasurable economic losses.At present,most researches focus on the physiological and biochemical characteristics of pathogenic bacteria and comprehensive prevention and treatment.The study of resistant microRNAs from the perspective of non-coding RNA has rarely been reported.In this paper,we aim to analyze the high-throughput sequencing data,namely small RNA sequencing technology,transcriptome sequencing technology,degradome sequencing technology,and joint analysis in the Panax notoginseng,to identify and microRNA-mediated microRNAs that are involved in the regulation of disease stress.The understanding of microRNAs in the regulation of medicinal plants and the development of related microRNA markers is expected to lay the foundation for high-quality Panax notoginseng breeding.The results of this project are as follows: 1.This study identified 277 microRNAs containing precursor sequences in response to black spot,of which 130 microRNAs containing precursor sequences have been reported,and 81 microRNAs containing precursor sequences are in 22 conserved microRNA families.2.SeqTar program,Cleaveland program for comparison,predicted conserved microRNA target genes,the results show that more accurate prediction results SeqTar program,which has been predicted total 123(base mismatches at most 4)in 20 conserved microRNA target family.3.In addition,7 pairs of negatively correlated conserved microRNAs and their target genes were obtained.The correlation between the negative correlations was verified by correlation PCR experiments.The target genes of the 7 pairs of conserved microRNAs were mostly ARF,GRF,CSD and other transcription factors.regulation of plant disease resistance response.This study is the first to use high-throughput multi-omics sequencing data(transcriptome sequencing,small RNA sequencing,degradome sequencing)to identify and analyze the function of microRNA in Panax notoginseng induced by black spot disease.Through the method developed by the non-coding RNA group of Kunming University of Science and Technology,the prediction and analysis were carried out to investigate the change of microRNA used in response to stress under the induction of black spot disease stress.For the first time,the resistance microRNA in the medicinal plant Panax notoginseng under disease stress was used as a molecular marker for resistance,which is expected to lay a foundation for screening high-quality Panax notoginseng breeding.