| [Camelina sativa(L.)Crantz] is a kind of oil crop with excellent characteristics.Flax mustard seeds are rich in a variety of unsaturated fatty acids,and its fatty acid composition is particularly suitable for human health.Long-term consumption of flax mustard oil products can reduce blood cholesterol content and some lowdensity lipoproteins,ensure cardiovascular health,and have broad prospects and development.Potential.Fatty acid desaturase-2(FAD2)that catalyzes the conversion of oleic acid to linoleic acid in the process of plant fatty acid biosynthesis and metabolism.The content and proportion of unsaturated fatty acids in plants are directly controlled by FAD2.And the quality of oil depends on the unsaturated degree of fatty acids in vegetable oils,so FAD2 gene has attracted extensive attention of scientists.It is of great significance to modify the quality of vegetable oils and fats by genetic engineering of FAD2 and adjusting the proportion of oleic acid and linoleic acid in plant seeds.In order to explore the function of CsFAD2 gene in flax mustard,we explore whether FAD2 gene regulates the synthesis of unsaturated fatty acids and what kind of fatty acids it regulates specifically,CsFAD2 gene was cloned from flax mustard seeds.The gene has a complete transcript of coding region with a length of 1155 bp.The expression vector pPICZalphaA-CsFAD2 of yeast was constructed and transformed into Pichia pastoris X33.After induction,the fatty acids were analyzed by gas chromatography(GC).The results showed that the content of saturated fatty acids and unsaturated fatty acids increased significantly,while the content of some fatty acids decreased significantly in the transgenic CsFAD2 gene compared with the control.For example,the content of γ-linolenic acid(C18.3N6)doubled from 38.87 mg/kg to 73.02 mg/kg.The content of oleic acid(C18.19C)decreased from 251.96 mg/kg to 30.22 mg/kg.This proves that CsFAD2 can promote the formation of linoleic acid from oleic acid and catalyze the synthesis of various fatty acids.It is of great significance to reveal the mechanism of fatty acid synthesis and metabolism,to improve the quality of oil and to cultivate new germplasm of flaxseed mustard. |
