Identification And Expression Analysis Of BrCNGCs And BcGRPs Genes Which Related To Resistance To TuMV In Cabbage

Chinese cabbage(Brassica rapa ssp.pekinensis)and non-heading Chinese cabbage(Brassica rapa ssp.chinensis Makino)were belong to Brassica rapa crops,they were widely cultivated vegetable in our country.They have important edible and economic value,however,due to the influence of plant diseases,the yield and quality of cabbage have been severely reduced.Therefore,it is of great significance to cultivate the varieties of cabbage with excellent resistance,and the identification and screeuing of disease-resistant genes is an important part.In this study,susceptible variety 'Chiifu-401-42' and '49 caixin' were used to study the disease resistent genes of Chinese cabbage and non-heading Chinese cabbage.The main results are as follows:1.The result of genome-wide identification of cyclic nucleotide gated ion channel genes(BrCNGCs)in Chinese cabbage showed there were 26 BrCNGCs in Chinese cabbage,and they can be classified into four major groups(?-?)and two subgroups(?a and ?b),they were predicted to be located on 9 chromosomes;gene structure analysis showed that 10 kinds of conserved motifs were identified in these genes;the results of quantitative RT-PCR showed that the interaction between plant growth regulator and TuMV can induce BrCNGCs high expression;the subcellular localization showed that BrCNGC7 protein distribution in cytoplasm and cell membrane.2.The BcGRP1 gene contained an open reading frame(ORF)with a length of 507 bp,encoding 168 amino acid,with a molecular formula of C1414H2323N507O612S94,a molecular weight of 39231.67 Da,and the IP of 5.23,belonged to the stable protein,had no transmembrane region and signal peptide region.The identity of BcGRP1 to the homologous genes in Arabidopsis and Chinese cabbage was more than 90%,the structural analysis showed that they were consisted of two exons and an intron.The N-terminal 9-82 amino acid of BcGRP1 protein contained a RNA recognition motif(RRM).The RRM domain contained two conserved ribonucleoprotein structures and named as RNP1 and RNP2,which are the active sites of RRM.BcGRP1 had closer relationship with homologous gene in Chinese cabbage,while the evolutionary relationship between BcGRP1 and homologous gene in maize was far.The qRT-PCR results showed that the BcGRP1 was induced by downy nildew and high temperature,while the expression level of BcGRP1 changed little under TuMV and NaCl treatment.Fifteen BcGRPs were identified in non-heading Chinese cabbage,the results of qRT-PCR showed that the expression level of gene 8?10?14 and 15 were significantly upregulated between 6 and 24 hours after the treatment of TuMV.