Expression Characteristics Analysis And Functional Verification Of Important Genes Of Tomato C2H2 Zinc Finger Protein Family Under Drought And Salt Stress

Changes in the natural environment have an important impact on the growth of tomatoes.Extreme environmental changes such as drought,salt and low temperature will reduce the yield and quality of tomatoes.Therefore,it is very important to develop tomato varieties with excellent stress resistance.Plant transcription factors play an important role in the process by which plants regulate their own adaptation to environmental changes,so they play an important role in many adversities.In the family of plant transcription factors,zinc finger protein is an important part of it.The QALGGH amino acid sequence unique to the C2H2 zinc finger protein can play a regulatory role in plant growth,especially in an adaptive response in adverse environment.Current research shows that this type of gene has an important regulatory effect on plants under drought and high salt stress.The team's previous experiments also found that these genes are actively expressed in drought and salt stress.Therefore,this study conducted a bioinformatics analysis of the C2H2 zinc finger protein family and ABA hormone-induced expression pattern analysis,VIGS technology was used to verify the resistance of the selected genes to disease and salt stress,which is the basis for the in-depth study of the gene resistance transformation mechanism of the family.The results show that:(1)Through the analysis of early transcriptome data,it was found that most of the C2H2 genes participated in the response under drought and salt stress.These genes were enriched in multiple molecular functions and cellular component GO classifications.KEGG analysis found that the gene family play a major role in resistance through glycosphingolipid biosynthesis-ganglio series,glycosaminoglycan degradation,sphingolipid metabolism,other glycan degradation,galactose metabolism and endocytosis.(2)The results showed that a number of significant response genes were obtained through screening,and comparative statistics found that 9 of them were transformed in response to salvage,10 were transformed in response to salt absorption,and 19 genes responded to both relief and salt absorption at the same time.Gene analysis results of stress-specific response showed that members involved in sugar metabolism-related pathways were mostly up-regulated during drought stress and down-regulated during salt stress;The members involved in the endocytosis pathway were only down-regulated under salt stress,and were not enriched under drought stress.(3)After the induction of exogenous ABA hormone,the 21 tested C2H2 genes all changed to varying degrees.Among them,the expression of Sl ZF-57 gene was up-regulated,while the genes of Sl ZF-18,Sl ZF-22,Sl ZF-24,Sl ZF-31,Sl ZF-35,Sl ZF-39,Sl ZF-63,Sl ZF-67 showed a trend of increasing first and then decreasing.The gene expression levels of Sl ZF-17,Sl ZF-20,Sl ZF-28,Sl ZF-30 and Sl ZF-68 were down-regulated.(4)According to the results of bioinformatics analysis,two combined down-significant responses and ABA hormone-induced up-regulated genes Sl ZF-31,Sl ZF-39,Sl ZF-57 were selected for functional verification.Down-regulated the expression of Sl ZF-31,Sl ZF-39 and Sl ZF-57 genes in tomato plants through gene silencing.The phenotypes of the silent plants treated by drought and salt stress were observed,and their gene expression patterns,physiological indexes,reactive oxygen staining and chlorophyll fluorescence parameters were analyzed.The results showed that the silencing of Sl ZF-31,Sl ZF-39 and Sl ZF-57 genes reduced the drought tolerance and salt tolerance of tomato plants to varying degrees.