Identification Of The Yellowhorn BZIP Transcription Factor Family And Expression Analysis In Response To Abiotic Stress

Yellowhorn is a woody oil crop unique to China.It could be extremely adaptable to environment and resistant to low temperature,drought,salinity and infertility.Since lack of genomic information,it’s very little research about molecular mechanisms in yellowhorn.The basic leucine zipper(bZIP)transcription factor family is one of the largest and most diverse families in plants,regulating plant growth and development and playing an essential role in response to abiotic and biotic stresses.Recently,the whole-genome sequence of yellowhorn has been completely sequenced,which provides an opportunity for comprehensive overview of the bZIP transcription factors in yellowhorn.This study firstly identified XsbZIP genes in the whole genome of yellowhom and systematically analyzed these genes.The main results are as follows:1.64 XsbZIP proteins were identified based on the information of whole-genome sequencing in yellowhorn.2.The phylogenetic tree was constructed according to phylogenetic relationships of XsbZIP,AtbZIP,ZmbZIP and GmbZIP transcription factors,and XsbZIP transcription factors were clustered into 11 groups(A to I,S and U).3.XsbZIP genes were divided into five types of patterns(a,b,c,d and e)on the basis of the intron number,position and splicing phase within the basic and hinge regions of the bZIP domains.4.20 conserved motifs were detected in XsbZIP proteins,and certain conserved motifs maybe confer specific functions on genes.5.Compared to tandem duplication,the segment duplication greatly contributed to the expansion of yellowhorn bZIP genes.6.62 XsbZIP genes with sequenced promoter regions harbor several stress and hormone responsive cis-elements.7.Most XsbZIP genes had different expression patterns after treatment with salt(NaCl),cold(4℃)and abscisic acid(ABA),speculating that XsbZIP genes were extensively involved in response to various abiotic stresses in yellowhorn.Based on gene expression patterns under abiotic stresses and functions of their collinear genes,we had predicted roles of XsbZIP11,XsbZIP21,XsbZIP25 and XsbZIP35 responded to various stresses in yellowhorn.