Effects Of Different Nitrogen Treatments On Lycium Barbarum Betaine And Its Key Stress Enzymes

In this test,"Ningqi No.1" was used as the test material,and the split zone test design was adopted.The main zone was nitrogen application depth A(0-20 cm,20-40 cm,40-60 cm),and the auxiliary zone was nitrogen application amount B(0 kg·667m-2,15 kg·667m-2,30 kg·667m-2 45 kg·667m-2,60 kg·667m-2),13 treatments,3 repetitions,a total of 39 plots.The effects of different nitrogen treatments on the indexes of Ningxia wolfberry betaine and Anti-stress key enzyme were explored,and the suitable nitrogen application amount and nitrogen application depth of the local wolfberry were selected.The main results are as follows:1.With the increase of nitrogen application rate,the B ADH content gradually decreases when the fertilization depth is 0-20cm,and at 20-40cm,the BADH and CMO content first increases and then decreases with the increase of the nitrogen application rate;the betaine content at the fertilization depth 0-20cm and 20-40cm,it decreases with the increase of nitrogen application.With the increase in the depth of fertilization,the betaine content will decrease in the green fruit period on June 3 and the end of summer fruit on July 19,and it will increase in the peak period of summer fruit;with the increase in the depth of fertilization,BADH and CMO The content reached the highest under A2 treatment.Among all the treatments,the content of betaine was the highest under the condition of A1B2 treatment,and the content of BADH and CMO was the highest under the condition of A2B3 treatment.2.Properly increasing the amount of nitrogen application(30 kg·667m-2,45 kg·667m-2)can increase the POD activity of medlar seven-inch branches and nutrient branches,and excessively deep fertilization(40-60cm)will reduce its activity;shallow fertilization to increase the amount of nitrogen application can increase the medlar seven-inch branches,Nutritional branch,Laoyan branch SOD activity.Among all the treatments,the SOD activity of the seven-inch branch was highest in the early stage of the results with A1B5 treatment,and the highest in the later stage of the treatment with A2B5;the SOD activity of the vegetative branch was highest with the treatment of A2B5 in the early stage,and the highest was A1B5 in the later stage;The highest under A2B5 processing conditions.3.Increasing the amount of nitrogen applied in shallow fertilization can increase the activity of POD,SOD and CAT of wolfberry fruit.SOD activity reached the highest when the nitrogen application rate was 30 kg·667m-2.In all treatments,the POD and CAT activities of fruits reached the highest under A1B5 treatment conditions.4.During the mature period of summer fruit,shallow fertilization and increased nitrogen application(45 kg·667m-2)can increase the chlorophyll content of wolfberry leaves,but the effect is not significant.The chlorophyll content of Qicun branches was higher than that of A1 and A2 in the early stage of fruit development,and higher than that of A3 in the condition of A1 and A2 in the ripening stage of summer fruit;A2 treatment is higher than A3 treatment.In all treatments,the chlorophyll content of medlar nutritional branches and chlorophyll was the highest under A1B4 treatment,and the chlorophyll content of Laoyan branch was the highest under A1B5 treatment.5.Betaine synthesis-related genes in wolfberry fruits were different among different treatments.The numbers of differentially expressed genes in A1B2,A1B3,A1B4,A1B5,A2B2,A2B3,A2B4,A2B5,A3B2,A3B3,A3B4,A3B5 were:4075,5908,7477,3225,6517,68475,767,7044,9888,6671,7826,and 7410.Among them,the differentially expressed genes involved in the betaine synthesis pathway were:126,55,54,56,56,53,61,62,73,58,73,66.It is presumed that A132 and A1B3 treatments could better regulate the expression of betaine synthesis-related genes in Lycium barbarum fruits,thereby affecting the betaine synthesis in Lycium barbarum fruits.6.Properly increasing the amount of nitrogen application is beneficial to increase the yield and fruit size of wolfberry.Deep fertilization will reduce the yield and particle size of wolfberry.When the fertilization depth is 0-20 cm and the nitrogen application amount is 45 kg·667m-2,the output of wolfberry reaches the maximum.The fruit size is the highest when the fertilization depth is 0-20cm and the nitrogen application amount is 60 kg·667m-2.