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Analysis On The Olfactory Receptor Genes And Identification Of Sex Pheromone Receptors In Cnaphalocrocis Medinalis Male Moths

Posted on:2021-03-06Degree:MasterType:Thesis
Country:ChinaCandidate:J J ChengFull Text:PDF
GTID:2393330611957342Subject:Plant protection
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In the long period of nature evolution,insects have evolved the highly sensitive olfactory system,which can sense various chemical compounds around environment.The compounds caused a series of electrophysiological reponse processed and integrated by peripheral and central nervous system,that decided insects behavior such as finding foods,habitats and partners,and escaping from prayers.Cnaphalocrocis medinalis is an important migratory insect pest of rice in China,which be used by disturbing and counting method(DCM)as monitoring method.However,it is labor cost and isn't accurate.Given the characteristics of folding leaf,pesticide control is not efficient.Thus we could use pheromone lure as monitoring method which also could control population density and reduce the utilize of pesticide.The sex pheromone of C.medinalis has been completely identified.But the function of the sex pheromone receptor genes has not been identified yet.Identifying the function of the sex pheromone receptor gene of C.medinalis could make contribution to mechanism of insects olfactory and provide theoretical basis about C.medinalis integrated management.The preliminary results are as follows:Three olfactory receptor genes of C.medinalis for detecting sex pheromone be obtained full-length sequence by Rapid Amplification of cDNA Ends(RACE)procedures.We analyzed the amino acid sequence,secondary structure and three-dimensional structure of those sex pheromone receptor genes and olfactory co-receptor(ORco).In the homology analysis of olfactory genes,we found that the PRs gene of C.medinalis developed into a single branch in the evolutionary tree,and the similarity of PRs genes reached to 49.62%,indicating that they may have similar functions in ligands.Functional verification of PRs was conducted using heterologous expression system of Xenopus laevis.CmedPR1 responded to Z11-18:OH and Z11-18:Ald.CmedPR2 responded to Z13-18:OH and Z13-18:Ald.CmedPR3 is receptor for detecting Z11-18:OH,Z13-18:OH.The results indicated that CmedPR2 and CmedPR3 ligands have a common compound: Z11-16:Ald,which not belonged to C.medinalis sex pheromone compounds.This study revealed the relationship between sex pheromone compounds and olfactory receptors,provide potential application in C.medinalis and important data for further research on the molecular mechanism of pheromone receptors.
Keywords/Search Tags:Cnaphalocrocis medinalis, sex pheromone, olfactory receptors, Olfactory gene analysis, two-electrode voltage-clamp
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