Genome-wide Association Analysis On The Infected Chicken By Salmonella Pullorum

Pullorum disease is a serious bacterial disease that threatens the modern poultry industry.This disease may result in great damages and economic losses.Thus far,the research on Salmonella pullorum(S.Pullorum)has focused mainly on the epidemiology and antigen-antibody reaction of bacteria,while the genetic basis of host infection and vertical transmission is still vague.In order to identify genes associated with S.Pullorum infection,we randomly selected 300 New-Pudong chickens as an experimental population,among which 150 hens were negative and 150 hens were positive,based on the results of whole-blood glass plate agglutination assay.The simplified genome sequencing method(dd-GBS)was performed,followed by a genome-wide association analysis(GWAS).After filtering,we obtain 314,562 high-quality genomic SNPs,which are evenly distributed across the whole genome.A total of 43 genome-wide significant SNP markers were found,located on chromosomes 1,2,3,5,7,13,17,and 20,respectively.Tweenty-one SNPs are focused on the interval of 57.7 Mb-59 Mb in the long arm of chromosome 5,where there is no known genes that are associated with bacterial infection and immunity,which implicates this region may be an important candidate for S.Pullorum infection.Furthermore,gene set enrichment analysis suggests 15 GO terms and 3 KEGG pathways are involved,including defense response to Gram-negative bacterium,B cell activation,vasoactive intestinal polypeptide receptor activity,alphaLinolenic acid metabolism,and so on.This work obtained SNPs markers,genes,candidate regions and pathways that associated with S.Pullorum infection on the basis of host's genome,which may provide a basic reference for population purification,prevention,and treatment of pullorum disease,enlightening the researches on mechanism of S.Pullorum infection and breeding for resistance.